マウスのインスリン型の成長因子2型受容体は,Tmeロカスにインプリントされ,密接に結びついています
D P Barlow1, R Stöger, B G Herrmann
1Research Institute for Molecular Pathology, Vienna, Austria.
Nature
|January 3, 1991
まとめ
母性効果変異Tmeはマウスの胚死を引き起こす. 研究者らはインスリン類似成長因子2型受容体 (Igf2r) 遺伝子がTmeにインプリントされ,リンクされていることを特定し,ゲノムインプリントに関する洞察を提供しました.
科学分野:
- 発達生物学 発達生物学とは
- 遺伝学 遺伝学とは
- ゲノミクスゲノミクスとは
背景:
- T関連母性効果 (Tme) は,マウスにおける唯一特定された母性効果変異であり,胚の致死につながる.
- 遺伝子の発現が親の起源に依存するゲノムインプリントは,マウスでは知られているが,インプリントされた遺伝子はほとんど特徴づけられていない.
- Tme場所の正確な位置は不明ですが,削除変異体を使用して染色体17の1cM領域に局所されています.
研究 の 目的:
- Tme locus.でインプリントされた遺伝子をクローンするために.
- Tme媒介の胚性致死性の遺伝的根拠を調査する.
主な方法:
- 染色体17の削除変異体 (ThpとtLub2) を利用して,Tmeの位置を狭めました.
- 遺伝子位置をマッピングするために,Igf2r,Sod-2,Plg,Tcp-1の探査機を用いたパルスフィールドゲル電泳を用いた.
- 発達中の胚における遺伝子発現パターン (母性対父性アレル) を分析した.
主要な成果:
- Tme locusは800~100 kbの領域に局限していた.
- インスリン型の成長因子型-2受容体 (Igf2r) とミトコンドリアのスーパーオキシドディスミュータゼ-2 (Sod-2) 遺伝子は,ThpとtLub2の2つのデリレーション変異体には存在しなかった.
- Igf2rは母性特異的な発現を示し,インプリントされていることを示し,TCP-1,Plg,Sod-2はバイアレル発現を示した.
結論:
- インスリン類似成長因子2型受容体 (Igf2r) 遺伝子はインプリントされ,Tmeロカスに非常に近い位置にあり,または同型である.
- この発見は,マウスにおける既知の母性効果変異に関連した最初のクローンインプリント遺伝子を提供する.
- Igf2rインプリントの理解は,Tmeに関連した胚性死亡のメカニズムとより広範なゲノムインプリントのプロセスに関する重要な洞察を提供します.
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