サイクリンは,ユビキチン経路によって分解される
M Glotzer1, A W Murray, M W Kirschner
1Department of Biochemistry, School of Medicine, University of California, San Francisco 94143-0448.
Nature
|January 10, 1991
まとめ
サイクリン分解は,破壊のためのタンパク質をマークすることによって,細胞サイクル進行を駆動します. このプロセスはユビキチン結合を含み,ユビキチン系がアナフェーズ発症を誘発することを示唆しています.
科学分野:
- 細胞生物学 細胞生物学
- 分子生物学は分子生物学である.
- バイオケミストリー バイオケミストリー
背景:
- サイクリン分解は,細胞循環の調節,特に脱離ミトーシスに不可欠です.
- サイクリン分解の分子メカニズムを理解することは,細胞サイクル制御を理解するための鍵です.
研究 の 目的:
- タンパク質の分解におけるサイクリンのN端の役割を調査する.
- ユビキチン依存型タンパク質分解によってサイクリン分解が起こるかどうかを判断する.
- ユビキチン系によるサイクリン認識とアナフェーズ誘発の間の潜在的な関連性を調査する.
主な方法:
- 異種タンパク質にサイクリンのN端部を融合させ,ハイブリッドタンパク質を構築する.
- ミトーシス中のハイブリッドタンパク質とネイティブサイクリンの分解パターンを分析する.
- ユビキチンとサイクリンとハイブリッドタンパク質の両方の結合を検証する.
- ユビキチン結合種の運動分析を行う.
主要な成果:
- サイクリンN末端を含むハイブリッドタンパク質は,ミトーシス中のタンパク質分解に敏感です.
- サイクリンとハイブリッドタンパク質は,分解時にユビキチンと結合し,結合を形成する.
- 動的データは,シクリンがユビキチン依存型タンパク質分解経路によって分解されることを強く示唆しています.
結論:
- サイクリンのN端領域には,ミトーシスでの分解のシグナルが含まれています.
- サイクリン分解は,ユビキチン結合系によって媒介されます.
- ユビキチン系によってサイクリンが認識されると,アナフェーズの発症を誘発する可能性がある.
関連する概念動画
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02:58Regulated Protein Degradation
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00:50Anaphase Promoting Complex
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02:58Regulated Protein Degradation
It is vital to regulate the activity of enzymatic as well as non-enzymatic proteins inside the cell. This can be achieved either through creating a balance between their rate of synthesis and degradation or regulating the intrinsic activity of the protein. Both these regulation mechanisms play an essential role in the normal functioning of cells.
Protein degradation plays two important roles in the cells. It helps to protect cells from misfolded or damaged proteins before they lead to a...
Protein degradation plays two important roles in the cells. It helps to protect cells from misfolded or damaged proteins before they lead to a...
00:50Anaphase Promoting Complex
The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...
01:13The Proteasome
Eukaryotic cells can degrade proteins through several pathways. One of the most important among these is the ubiquitin-proteasome pathway. It helps the cell eliminate the misfolded, damaged, or unwarranted cytoplasmic proteins in a highly specific manner.
In this pathway, the target proteins are first tagged with small proteins called ubiquitin. This involves participation of a series of enzymes including— E1 (ubiquitin-activating enzyme), E2 (ubiquitin-conjugating enzyme), and E3 (ubiquitin...
In this pathway, the target proteins are first tagged with small proteins called ubiquitin. This involves participation of a series of enzymes including— E1 (ubiquitin-activating enzyme), E2 (ubiquitin-conjugating enzyme), and E3 (ubiquitin...