肝臓のフィブロブラスト成長因子受容体は,3つの構造領域で異なる

J Z Hou¹, M K Kan, K McKeehan

¹W. Alton Jones Cell Science Center, Inc., Lake Placid, NY 12946.

Science (New York, N.Y.)

|February 8, 1991

まとめ

ヒトの肝細胞における線維芽生殖成長因子 (FGF) 受容体変種は,多様な構造を示している. キナーゼ活性差を含むこれらの変異は,肝臓の再生と肝腫の発達に影響を与える可能性があります.

科学分野:

分子生物学は分子生物学である.
細胞生物学細胞生物学
遺伝学遺伝学とは

背景:

ヘパリン結合性フィブロブラスト成長因子 (FGF) 信号伝達は,肝臓の再生と肝腫にとって極めて重要です.
ヒトの肝細胞における特定の受容体変種とその機能は,まだ完全に理解されていません.

研究の目的:

ヒト肝細胞における線維芽生殖成長因子 (FGF) 受容体変異体の構造的多様性と潜在的な機能的影響を調査する.

主な方法:

タンパク質の構造を予測するために,補完的なDNAヌクレオチド配列の分析.
FGF受容体ファミリーのメンバー内の保存モチーフの識別.

主要な成果:

1つのヒト肝細胞集団から少なくとも6〜12の同類のFGF受容体ポリペプチドが予測されています.
識別されたアミノ末端の変種は,免疫グロブリンのような異硫化物ループが異なるトランスメブランおよび細胞内形態を含む.
潜在的なセリン・スレオニンキナーゼ部位に基づいて,異なるジュークスタメンブランのモチーフ.
特徴的なカルボキシル末端のモチーフ,一方は推論的なチロシンキナーゼ活性とリン酸化部位を持ち,もう一方はこれらの特徴を欠いている.

結論:

人間の肝細胞は,構造的に有意な異質性を有するFGF受容体の複雑な家族を表現する.
FGF受容体のキナーゼ活性と構造ドメインの変動は,肝臓の再生と肝臓腫の病原化に役割を果たす可能性があります.

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