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関連する概念動画

Ligand Binding and Linkage00:49

Ligand Binding and Linkage

Allosteric proteins have more than one ligand binding site; the binding of a ligand to any of these sites influences the binding of ligands to the other sites. When a protein is allosteric, its binding sites are called coupled or linked.  In the case of enzymes, the site that binds to the substrate is known as the active site and the other site is known as the regulatory site. When a ligand binds to the regulatory site, this leads to conformational changes in the protein that can influence the...
Enzymes02:34

Enzymes

Inside living organisms, enzymes act as catalysts for many biochemical reactions involved in cellular metabolism. The role of enzymes is to reduce the activation energies of biochemical reactions by forming complexes with its substrates. The lowering of activation energies favor an increase in the rates of biochemical reactions.
Enzyme deficiencies can often translate into life-threatening diseases. For example, a genetic abnormality resulting in the deficiency of the enzyme G6PD...
Induced-fit Model01:13

Induced-fit Model

Most chemical reactions in cells require enzymes—biological catalysts that speed up the reaction without being consumed or permanently changed. They reduce the activation energy needed to convert the reactants into products. Enzymes are proteins, that usually work by binding to a substrate—a reactant molecule that they act upon.
Enzymes exhibit substrate specificity, meaning that they can only bind to certain substrates. This is mainly determined by the shape and chemical characteristics of...
Ligand Binding Sites02:40

Ligand Binding Sites

Proteins are dynamic macromolecules that carry out a wide variety of essential processes; however, the activities of most proteins depend on their interactions with other molecules or ions, known as ligands.
Protein-ligand interactions are quite specific; even though numerous potential ligands surround a cellular protein at any given time, only a particular ligand can bind to that protein. Moreover, a ligand binds only to a dedicated area on the surface of the protein, known as the...
Introduction to Mechanisms of Enzyme Catalysis01:13

Introduction to Mechanisms of Enzyme Catalysis

For many years, scientists thought that enzyme-substrate binding took place in a simple "lock-and-key" fashion. This model stated that the enzyme and substrate fit together perfectly in one instantaneous step. However, current research supports a more refined view scientists call induced fit. The induced-fit model expands upon the lock-and-key model by describing a more dynamic interaction between enzyme and substrate. As the enzyme and substrate come together, their interaction causes a mild...
SN1 Reaction: Kinetics02:05

SN1 Reaction: Kinetics

In an SN2 reaction, the reaction rate depends on both the type of nucleophile and the substrate. A hindered tertiary alkyl halide is practically inert to the SN2 mechanism despite using a strong nucleophile.
However, Sir Christopher Ingold and Edward D. Hughes, who studied the kinetics of various nucleophilic substitution reactions, noticed that a tertiary alkyl halide does undergo a nucleophilic substitution reaction in the presence of a weak nucleophile. While studying the substitution...

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Preparation of SNS Cobalt(II) Pincer Model Complexes of Liver Alcohol Dehydrogenase
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Published on: March 19, 2020

シバサキのLi3(THF) n(BINOLate) 3Ln複合体における基板結合に関する洞察と,触媒作用における影響について.

Alfred J Wooten1, Patrick J Carroll, Patrick J Walsh

  • 1P. Roy and Diana T. Vagelos Laboratories, Department of Chemistry, University of Pennsylvania, 231 South 34th Street, Philadelphia, Pennsylvania 19104-6323, USA.

Journal of the American Chemical Society
|May 16, 2008
PubMed
まとめ

この研究は,新しい異金属ルイス酸の特徴を明らかにし,ランタニドセンターへの基板結合が非対称な触媒にどのように影響するかを明らかにしました. 発見は,ランタニドセンターがバイデンタート基板をケラートしない可能性を示唆し,触媒反応におけるエナチオ選択性に影響を与えます.

さらに関連する動画

Bio-layer Interferometry for Measuring Kinetics of Protein-protein Interactions and Allosteric Ligand Effects
13:57

Bio-layer Interferometry for Measuring Kinetics of Protein-protein Interactions and Allosteric Ligand Effects

Published on: February 18, 2014

Catalytic Reactions at Amine-Stabilized and Ligand-Free Platinum Nanoparticles Supported on Titania During Hydrogenation of Alkenes and Aldehydes
12:08

Catalytic Reactions at Amine-Stabilized and Ligand-Free Platinum Nanoparticles Supported on Titania During Hydrogenation of Alkenes and Aldehydes

Published on: June 24, 2022

関連する実験動画

Last Updated: Jul 5, 2026

Preparation of SNS Cobalt(II) Pincer Model Complexes of Liver Alcohol Dehydrogenase
06:31

Preparation of SNS Cobalt(II) Pincer Model Complexes of Liver Alcohol Dehydrogenase

Published on: March 19, 2020

Bio-layer Interferometry for Measuring Kinetics of Protein-protein Interactions and Allosteric Ligand Effects
13:57

Bio-layer Interferometry for Measuring Kinetics of Protein-protein Interactions and Allosteric Ligand Effects

Published on: February 18, 2014

Catalytic Reactions at Amine-Stabilized and Ligand-Free Platinum Nanoparticles Supported on Titania During Hydrogenation of Alkenes and Aldehydes
12:08

Catalytic Reactions at Amine-Stabilized and Ligand-Free Platinum Nanoparticles Supported on Titania During Hydrogenation of Alkenes and Aldehydes

Published on: June 24, 2022

科学分野:

  • 有機金属化学 有機金属化学
  • アシンメトリック・カタリシス
  • ランタナイド化学 ランタナイド化学

背景:

  • ヘテロビメタリックルイス酸,特にLi3 (((THF) n (((BINOLate) 3Lnは,高エナチオ選択性を持つ強力な非対称な触媒です.
  • 触媒と基板の相互作用と作用の正確なメカニズムは,まだ完全に理解されていません.

研究 の 目的:

  • 新しい異金属複合体を分離し,構造的に特徴づける.
  • 溶液結合研究とNMRスペクトロスコピーを用いて,触媒-基板相互作用を調査する.
  • 触媒活性と選択性におけるランタニドとリチウムセンターの役割を明らかにする.

主な方法:

  • Li3(THF) n(BINOLate) 3LnとLi3(py) 5(BINOLate) 3Ln複合体の分離とX線構造的特徴付けについて
  • ルイス塩基基底類 (サイクロヘクセノン,DMF,ピリジン) を用いた溶液結合研究.
  • ランタニド誘発シフト (LIS) NMRスペクトロスコーピーは,パラ磁性ランタニド複合体を使用します.
  • Li3 ((DMEDA) 3 ((BINOLate) 3Ln複合体の構造と溶液に関する研究.

主要な成果:

  • 異なるリガンド (THF,ピリジン) を有する7および8座標ヘテロメタリック複合体の構造的特徴.
  • 溶液中のランタニド中枢に基板アナログの結合が実証された.
  • N,N'-ジメチルエチレンダイアミン (DMEDA) がリチウム中心に選択的に結合し,ダイアステロエメリックに純粋な複合体を形成する.
  • DMEDAアダクトを使用した触媒非対称反応は,既存の触媒と比較できるエナチオ選択性を得ました.

結論:

  • Li3(THF) n(BINOLate) 3Ln複合体のランタニドセンターは,バイデント酸塩基をケラートしないという仮説を立てた.
  • Li3(THF) n(BINOLate) 3La触媒の高いエナチオ選択性を説明するメカニズムを提案した.
  • 効果的な非対称な触媒としてのDMEDA添加物の可能性を強調した.