関連する実験動画
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Detection of Protein Ubiquitination
Published on: August 19, 2009
DeTEKingは,APC/C基板のユビキチネーションを検出する
Arthur L Haas1, Keith D Wilkinson
1Department of Biochemistry and Molecular Biology, Louisiana State University School of Medicine, New Orleans, LA 70112, USA.
Cell
|May 20, 2008
まとめ
細胞分裂には,ユビキチン-プロテアソーム系を通じたタンパク質の制御された分解が不可欠である. 新しく特定されたTEKモチーフは,ミトーシス中の標的型タンパク質破壊のためにポリユビキチン鎖を組み立てるのに役立ちます.
科学分野:
- 分子生物学は分子生物学である.
- 細胞生物学 細胞生物学
- バイオケミストリー バイオケミストリー
背景:
- ユビキチン-プロテアソーム経路は,タンパク質の分解を調節し,細胞サイクル進行を保証します.
- アナフェーズ促進複合体/サイクロソーム (APC/C) ユビキチンリガゼは,ポリユビキチン鎖を付着させることで,タンパク質を分解する標的となる.
- これらのポリユビキチン鎖は,破壊のために26Sプロテアソームによって認識されます.
研究 の 目的:
- APC/Cがポリユビキチン分解信号の組み立てを媒介する分子機構を特定する.
- APC/C.による基質認識とユビキチネーションにおける特定のモチーフの役割を明らかにする.
主な方法:
- この研究では,タンパク質とタンパク質の相互作用を研究するための生化学的測定法が含まれていた可能性が高い.
- 実験室内ユビキチネーションアッセイ (in vitro ubiquitination assays) や潜在的に構造生物学的な方法などの技術が用いられた可能性がある.
- APC/C.による基板およびユビキチン改変の分析.
主要な成果:
- APC/C基板とユビキチンそのものに存在する新しいTEKモチーフの特定.
- TEKモチーフは,APC/C標的のポリユビキチン鎖の組み立てを媒介することが示されました.
- このモチーフは,細胞循環調節体の認識と分解に不可欠です.
結論:
- TEKモチーフは,APC/C媒介のユビキチネーションプロセスの重要な構成要素です.
- この発見は,制御されたタンパク質の分解が細胞サイクル進行をどのように推進するかについてより深い理解を提供します.
- この発見は,細胞サイクル関連の疾患における治療的介入の潜在的標的を提供している.
関連する概念動画
Covalently Linked Protein Regulators
Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein.
These groups modify specific amino acids in a protein.
The Proteasome
Eukaryotic cells can degrade proteins through several pathways. One of the most important amongst these is the ubiquitin-proteasome pathway. It helps the cell eliminate the misfolded, damaged, or unwarranted cytoplasmic proteins in a highly specific manner.
In this pathway, the target proteins are first tagged with small proteins called ubiquitin. A series of enzymes carry out the ubiquitination of the target proteins - E1 (ubiquitin-activating enzyme), E2 (ubiquitin-conjugating enzyme), and E3...
In this pathway, the target proteins are first tagged with small proteins called ubiquitin. A series of enzymes carry out the ubiquitination of the target proteins - E1 (ubiquitin-activating enzyme), E2 (ubiquitin-conjugating enzyme), and E3...
Regulated Protein Degradation
It is vital to regulate the activity of enzymatic as well as non-enzymatic proteins inside the cell. This can be achieved either through creating a balance between their rate of synthesis and degradation or regulating the intrinsic activity of the protein. Both these regulation mechanisms play an essential role in the normal functioning of cells.
Protein degradation plays two important roles in the cells. It helps to protect cells from misfolded or damaged proteins before they lead to a...
Protein degradation plays two important roles in the cells. It helps to protect cells from misfolded or damaged proteins before they lead to a...
Anaphase Promoting Complex
The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...
Anaphase Promoting Complex
The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...
The Proteasome
Eukaryotic cells can degrade proteins through several pathways. One of the most important among these is the ubiquitin-proteasome pathway. It helps the cell eliminate the misfolded, damaged, or unwarranted cytoplasmic proteins in a highly specific manner.
In this pathway, the target proteins are first tagged with small proteins called ubiquitin. This involves participation of a series of enzymes including— E1 (ubiquitin-activating enzyme), E2 (ubiquitin-conjugating enzyme), and E3 (ubiquitin...
In this pathway, the target proteins are first tagged with small proteins called ubiquitin. This involves participation of a series of enzymes including— E1 (ubiquitin-activating enzyme), E2 (ubiquitin-conjugating enzyme), and E3 (ubiquitin...

