Rad6-Rad18は,9-1-1チェックポイントクランプをユビキチン化することによって,真核生物のSOS応答を媒介する

Yu Fu¹, Yu Zhu, Ke Zhang

¹Department of Microbiology and Immunology, University of Saskatchewan, Saskatoon, SK, S7N 5E5, Canada.

Cell

|May 20, 2008

まとめ

Rad6-Rad18複合体は,バクテリアのSOS応答に似た酵母におけるDNA損傷反応を調整する. タンパク質のユビキチネーションを通じて,遺伝子転写とDNA修復経路を調節する.

科学分野:

分子生物学は分子生物学である.
遺伝学遺伝学とは
バイオケミストリーバイオケミストリー

背景:

ユカリオットは,細菌のSOS応答に似た,明確に定義された全局的なDNA損傷応答を欠いている.
Rad6-Rad18ユビキチネーション複合体は,DNA修復に関与することが知られている.

研究の目的:

ユカリオットDNA損傷反応におけるRad6-Rad18複合体の役割を調査する.
Rad6-Rad18がDNA損傷後のグローバルな遺伝子転写に影響を与えるかどうかを判断する.

主な方法:

イースト遺伝学と分子生物学技術.
DNA損傷に対する反応として遺伝子発現の変化の分析.
ウビキチネーションアッセイとタンパク質相互作用の研究.

主要な成果:

Rad6-Rad18複合体は,多数の酵母遺伝子のDNA損傷誘発の転写上調節に不可欠である.
Rad6-Rad18は,K197.7でRad17 (9-1-1複合体のサブユニット) のモノウビキチネーションを触媒化する.
Rad17のユビキチネーションは,効率的なRad53のリン酸化とDNA損傷チェックポイントの活性化に不可欠です.

結論:

Rad6-Rad18複合体は,バクテリアのSOS応答に類似して,酵母におけるDNA損傷反応の間に,グローバル遺伝子発現の重要な調節体として作用する.
この複合体は,Ubiquitylating DNA クランプ PCNA と 9-1-1 を使って,複数の DNA 損傷反応経路を調整しています.

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