Jove
Visualize
お問い合わせ
JoVE
x logofacebook logolinkedin logoyoutube logo
JoVEについて
概要リーダーシップブログJoVEヘルプセンター
著者向け
出版プロセス編集委員会範囲と方針査読よくある質問投稿
図書館員向け
推薦の声購読アクセスリソース図書館諮問委員会よくある質問
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experimentsアーカイブ
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教員リソースセンター教員サイト
利用規約
プライバシーポリシー
ポリシー

関連する概念動画

Molecular Spectroscopy: Absorption and Emission01:14

Molecular Spectroscopy: Absorption and Emission

Molecules possess discrete energy levels called quantum states. Unlike atoms, which have simpler energy levels, molecules possess additional rotational and vibrational energy levels. Each energy level is separated by an energy gap, with the gaps between adjacent electronic, vibrational, and rotational levels varying significantly. The three types of energy levels in a diatomic molecule are shown in Figure 1.
UV–Vis Spectroscopy: Molecular Electronic Transitions01:16

UV–Vis Spectroscopy: Molecular Electronic Transitions

In Ultraviolet–Visible (UV–Vis) spectroscopy, the absorption of electromagnetic radiation is used to probe the electronic structure of molecules. This technique provides insights into molecular electronic transitions, particularly the movement of electrons between different molecular orbitals. Radiation is absorbed if the energy of the electromagnetic radiation passing through the molecule is precisely equal to the energy difference between the excited and ground states. During this process,...
Electron Paramagnetic Resonance (EPR) Spectroscopy: Organic Radicals01:17

Electron Paramagnetic Resonance (EPR) Spectroscopy: Organic Radicals

Ideally, an unpaired electron shows a single peak in the EPR spectrum due to the transition between the two spin energy states. However, coupling interactions can occur between the spins of the unpaired electron and any neighboring spin-active nuclei. This hyperfine coupling results in hyperfine splitting, where the EPR signal is split into multiplets. The signals split into 2nI + 1 peaks, where n is the number of equivalent nuclei and I is the nuclear spin. These splitting patterns provide...
Single-Strand DNA Binding Proteins01:03

Single-Strand DNA Binding Proteins

For successful DNA replication, the unwinding of double-stranded DNA must be accompanied by stabilization and protection of the separated single strands of the DNA. This crucial task is performed by single-strand DNA-binding (SSB) proteins. They bind to the DNA in a sequence-independent manner, which means that the nitrogenous bases of the DNA need not be present in a specific order for binding of SSB proteins to it. The binding of SSB proteins straightens single-stranded DNA (ssDNA) and makes...
Fixing Double-strand Breaks02:04

Fixing Double-strand Breaks

The double-stranded structure of DNA has two major advantages. First, it serves as a safe repository of genetic information where one strand serves as the back-up in case the other strand is damaged. Second, the double-helical structure can be wrapped around proteins called histones to form nucleosomes, which can then be tightly wound to form chromosomes. This way, DNA chains up to 2 inches long can be contained within microscopic structures in a cell. A double-stranded break not only damages...
DNA as a Genetic Template02:05

DNA as a Genetic Template

Two structural features of the DNA molecule provide a basis for the mechanisms of heredity: the four nucleotide bases and its double-stranded nature. The Watson-Crick model of double-helical DNA structure, proposed in 1952, drew heavily upon the X-ray crystallography work of researchers Rosalind Franklin and Maurice Wilkins. Watson, Crick, and Wilkins jointly received the Nobel Prize in Physiology or Medicine for their work in 1962. Franklin was, controversially, excluded from the prize for...

こちらも読む

関連記事

共著者、ジャーナル、引用グラフによってこの研究に関連する記事。

並び替え
Same author

Regiospecific <i>N</i>-alkyl substitution tunes the molecular packing of high-performance non-fullerene acceptors.

Materials horizons·2021
Same author

Source of Bright Near-Infrared Luminescence in Gold Nanoclusters.

ACS nano·2021
Same author

Enhancing Entangled Two-Photon Absorption for Picosecond Quantum Spectroscopy.

Journal of the American Chemical Society·2021
Same author

Quasiclassical Trajectory Study of the O(<sup>3</sup>P) + CO<sub>2</sub>(<sup>1</sup>Σ<sub>g</sub><sup>+</sup>) Reaction at Hyperthermal Energies.

The journal of physical chemistry. A·2021
Same author

Charge transport through extended molecular wires with strongly correlated electrons.

Chemical science·2021
Same author

Plasmonic Nanoparticle Lattice Devices for White-Light Lasing.

Advanced materials (Deerfield Beach, Fla.)·2021

関連する実験動画

Updated: Jul 5, 2026

CD Spectroscopy to Study DNA-Protein Interactions
06:48

CD Spectroscopy to Study DNA-Protein Interactions

Published on: February 10, 2022

単一鎖DNAの電子刺激とスペクトル

Stefano Tonzani1, George C Schatz

  • 1Department of Chemistry, Northwestern University, Evanston, Illinois 60208-3113, USA. tonzani@northwestern.edu

Journal of the American Chemical Society
|May 22, 2008
PubMed
まとめ

3つの塩基を網羅する分散電子状態は,単一鎖DNAで直接刺激された. この発見は,強力な塩基堆積でも,DNAの移位長に関する実験データと一致しています.

科学分野:

  • 計算化学はコンピュータ化学である.
  • バイオフィジックス 生物物理学
  • 分子ダイナミクス 分子ダイナミクス

背景:

  • DNAの電子的性質を理解することは,分子電子やセンシングなどの分野にとって極めて重要です.
  • 以前の研究では,DNAにおける電荷輸送と非局所化が研究されているが,単一鎖DNAにおける非局所化状態の直接刺激は,依然として活発な研究分野である.

研究 の 目的:

  • 単一鎖のポリネタ (A) DNAにおける非局所化された電子状態の直接刺激を,計算的方法を用いて調査する.
  • シミュレーション結果を,移位長と円形の二重光スペクトルの実験データと比較する.

主な方法:

  • 密度関数理論 (DFT) の計算.密度関数理論 (DFT) の計算.密度関数理論 (DFT) の計算.密度関数理論 (DFT) の計算.密度関数理論 (DFT) の計算.密度関数理論 (DFT) の計算.
  • 分子ダイナミクス (MD) シミュレーション.
  • 計算された円形の二重光 (CD) スペクトルを用いて構造モデルの検証.

主要な成果:

  • 3つの塩基に及ぶ非局所化状態の直接刺激は,単一鎖のポリネタ (A) DNAで観察されました.
  • デロカライゼーションの長さのシミュレーション結果は,単一および二重鎖DNAの両方の実験結果と半量的な一致を示しました.

さらに関連する動画

Spectroscopic Super-resolution Imaging of DNA Molecules using Intrinsic Contrast
09:19

Spectroscopic Super-resolution Imaging of DNA Molecules using Intrinsic Contrast

Published on: March 6, 2026

Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes
07:44

Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes

Published on: July 6, 2016

関連する実験動画

Last Updated: Jul 5, 2026

CD Spectroscopy to Study DNA-Protein Interactions
06:48

CD Spectroscopy to Study DNA-Protein Interactions

Published on: February 10, 2022

Spectroscopic Super-resolution Imaging of DNA Molecules using Intrinsic Contrast
09:19

Spectroscopic Super-resolution Imaging of DNA Molecules using Intrinsic Contrast

Published on: March 6, 2026

Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes
07:44

Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes

Published on: July 6, 2016

  • 短いDNAオリゴーマー (d(A) 2とd(A) 4) の計算されたCDスペクトルは実験データと一致し,シミュレートされた構造の妥当性を確認しました.

    • 結論:

    • この研究は,単一鎖DNAにおける三塩基非局所化状態の存在と直接刺激を確認した.
    • この発見は,単一鎖DNAにおける有意な塩基の積み重ねが,短距離の異地化を排除しないことを示唆している.
    • 採用された計算方法は,DNAの電子特性と構造動態を研究するための信頼性の高いアプローチを提供します.