関連する実験動画
Updated: May 10, 2026

11:03
Conditional Genetic Transsynaptic Tracing in the Embryonic Mouse Brain
Published on: December 22, 2014
trkプロトオンコゲンは,神経成長因子の受容体をコードする
1Department of Molecular Biology, Bristol-Myers Squibb Pharmaceutical Research Institute, Princeton, New Jersey 08543-4000.
Cell
|April 5, 1991
まとめ
trkプロトオンコゲン産物であるgp140trkは,高親和性神経成長因子 (NGF) 受容体として機能する. この受容体はNGFシグナル伝達を介し,チロシンリン酸化とキナーゼ活性を含み,非神経細胞の低親和性受容体とは独立しています.
科学分野:
- 神経科学は神経科学である.
- 分子生物学は分子生物学である.
- 細胞シグナル伝達 細胞信号伝達
背景:
- 2つの異なる神経成長因子 (NGF) 受容体が存在します:低親和性受容体 (LNGFR) と高親和性受容体の構造は不明です.
- 低親和性受容体は,LNGFR遺伝子によってコードされたシステインに富んだグリコタンパク質である.
研究 の 目的:
- 高親和性NGF受容体の構造的性質を特定するために.
- NGF結合とシグナル伝達におけるtrkプロトオンコゲン産物 (gp140trk) の機能的役割を調査する.
主な方法:
- NGFがgp140trkに結合することを検出するための化学クロスリンク実験.
- 様々な細胞タイプ (PC12,マウスの線維芽細胞,昆虫のSf9細胞) でgp140trkの発現分析.
- NGF刺激によるgp140trkチロシンリン酸化とチロシンキナーゼ活性に関する評価.
主要な成果:
- ヒトトーク原発がん遺伝子の産物であるgp140trkは,NGFを高親和度 (Kd ~ 10^-11 から 10^-10 M) で結合する.
- NGFは,LNGFRが欠けている細胞を含む複数の細胞タイプでgp140trkにクロスリンクすることができます.
- NGFの刺激により,急速なチロシンリン酸化が誘発され,gp140trkのチロシンキナーゼ活性が活性化されます.
結論:
- gp140trkは,機能的な高親和性NGF受容体である.
- gp140trkは,チロシンキナーゼの活性化を含むNGF信号伝導経路を媒介する.
- gp140trkへの高親和性NGF結合は,神経細胞以外の細胞でLNGFRとは独立して起こる可能性があります.
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