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Introduction to Nuclear Reprogramming01:14

Introduction to Nuclear Reprogramming

Nuclear reprogramming is the process of switching gene expression of one cell type to that of another cell type, usually from a differentiated cell state to an undifferentiated cell state. Differentiation occurs during processes such as development and morphogenesis, tissue regeneration, and malignancy. Cells can also be artificially induced to reprogram their gene expression by techniques such as nuclear transfer, induced pluripotency, and cell fusion. Such techniques have many applications in...
Somatic to iPS Cell Reprogramming01:29

Somatic to iPS Cell Reprogramming

Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012 for this...

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In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression
08:54

In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression

Published on: March 29, 2019

統合的ゲノム分析を通じて直接再プログラミングを解剖する.

Tarjei S Mikkelsen1, Jacob Hanna, Xiaolan Zhang

  • 1Broad Institute of MIT and Harvard, 7 Cambridge Center, Cambridge, Massachusetts 02142, USA.

Nature
|May 30, 2008
PubMed
まとめ
この要約は機械生成です。

ソマティック細胞をプラリポテンシーに再プログラムするには,複雑な遺伝子発現の変化が含まれます. 部分的に再プログラムされた細胞は不完全な因子抑制と非効率的なDNA脱メチル化により閉じ込められるが,RNA阻害および脱メチル化阻害剤は効率を改善する.

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Amplification, Next-generation Sequencing, and Genomic DNA Mapping of Retroviral Integration Sites
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Amplification, Next-generation Sequencing, and Genomic DNA Mapping of Retroviral Integration Sites

Published on: March 22, 2016

関連する実験動画

Last Updated: Jul 4, 2026

In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression
08:54

In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression

Published on: March 29, 2019

Bidirectional Retroviral Integration Site PCR Methodology and Quantitative Data Analysis Workflow
12:53

Bidirectional Retroviral Integration Site PCR Methodology and Quantitative Data Analysis Workflow

Published on: June 14, 2017

Amplification, Next-generation Sequencing, and Genomic DNA Mapping of Retroviral Integration Sites
09:31

Amplification, Next-generation Sequencing, and Genomic DNA Mapping of Retroviral Integration Sites

Published on: March 22, 2016

科学分野:

  • * 細胞の再プログラムと発達生物学.
  • * 細胞運命を変化させるゲノムとエピジェネティック分析.

背景:

  • *体細胞は,子宮外転写因子発現を通じて多能性を取り戻すことができる.
  • *再プログラムメカニズムを理解することは,効率と安全性を向上させるための鍵です.

研究 の 目的:

  • * マウスの線維芽細胞とBリンパ球の再プログラムに関する統合的ゲノム分析を行う.
  • * 細胞が多能性を再プログラムするメカニズムと運動を研究する.

主な方法:

  • * マウスの線維芽細胞とリプログラミング中のBリンパ球の統合的ゲノム分析.
  • *遺伝子発現,表遺伝子状態,転写因子の活性に関する分析.

主要な成果:

  • * 完全に再プログラムされた細胞は,胚性幹細胞のような遺伝子発現と表遺伝子プロファイルを達成します.
  • * 部分的に再プログラムされた細胞は,不完全な転写因子抑制とDNAハイパーメチル化が重要な位置で示される.
  • * 不完全な抑制と非効率的なDNA脱メチル化は完全な再プログラミングを妨げます.

結論:

  • * 細胞は,不完全な転写因子静止により,部分的に再プログラムされた状態に閉じ込められる.
  • *DNA脱メチル化は,多能性を達成する上で重要な,しかし非効率的なステップです.
  • *RNA阻害およびDNAメチルトランスファーゼ阻害剤は,再プログラム効率を高めます.