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Updated: Jul 4, 2026

Efficient and Site-specific Antibody Labeling by Strain-promoted Azide-alkyne Cycloaddition
09:06

Efficient and Site-specific Antibody Labeling by Strain-promoted Azide-alkyne Cycloaddition

Published on: December 23, 2016

サイト特有の (18) タンパク質ホルモンレプチンのFラベル付けは,一般的な2段階の結合手順を用いて行われます

Robert R Flavell1, Paresh Kothari, Maya Bar-Dagan

  • 1Laboratory of Synthetic Protein Chemistry, Laboratory of Molecular Genetics, Howard Hughes Medical Institute, The Rockefeller University, New York, New York 10065, USA.

Journal of the American Chemical Society
|June 24, 2008
PubMed
まとめ

研究者らは,レプチンホルモンの放射性トレーサーを作成する新しい方法を開発し,体内の分布の詳細なイメージングを可能にしました. このテクニックは,ポジトロン放出トモグラフィー (PET) を使用することで,レプチン抵抗性および肥満を理解するのに役立ちます.

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Tagging and Fusion Proteins01:24

Tagging and Fusion Proteins

Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...
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Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...