DSCAMはネトリン受容体であり,ネトリン-1への回転反応を媒介するためにDCCと連携する
Alice Ly1, Anatoly Nikolaev, Geetha Suresh
1Department of Molecular, Cellular, and Developmental Biology, Yale University, 266 Whitney Avenue, New Haven, CT 06520, USA.
Cell
|July 1, 2008
まとめ
ダウン ダウン ダウン
科学分野:
- 神経科学は神経科学である.
- 発達生物学 発達生物学とは
- 分子生物学は分子生物学である.
背景:
- 脊椎弁関節軸索は,神経系の発達中に腹中線を移動する.
- Netrin-1とその受容体DCCは,既知の誘導信号であるが,追加の成分が疑われる.
- ダウン症候群の細胞粘着分子 (DSCAM) は,このプロセスのための候補遺伝子です.
研究 の 目的:
- DSCAMが脊髄後関節軸索の誘導に果たす役割を調査する.
- DSCAMがネットリン-1受容体として機能するかどうかを判断する.
- ネトリン-1とDSCAMを含むシグナル伝達経路を解明する.
主な方法:
- DSCAMの脊髄後関節軸索における発現分析.
- DSCAMに対するネットリン-1結合測定法.
- 遺伝子操作を用いた機能的研究 in vivo および in vitro.
- DSCAMによって媒介されるネトリン-1の引き寄せをテストするために,Xenopus脊髄神経細胞の拡張培養.
主要な成果:
- DSCAMは,脊髄後関節軸索に発現し,ネトリン-1と結合する.
- DSCAMは,軸索が静脈の中央線に向かって,そして横に成長するために不可欠です.
- DSCAMとDCCの両方が,ネットリン-1に対するニューロンの回転反応を独立して媒介することができます.
- Xenopusの神経細胞における外因的なDSCAM発現は,DCCとは独立して,ネトリン-1の引き寄せを与える.
結論:
- DSCAMは,ネトリン-1の機能的受容体として作用する.
- ネトリン-1/DSCAMシグナル伝達は,脊椎動物のコンシューラルの軸索誘導において重要な役割を果たします.
- DSCAMは,神経系の発達中のミッドライン軸索経路発見の重要な媒介である.
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