G1サイクリンのポジティブなフィードバックは,一貫した細胞サイクルエントリーを確保します

Jan M Skotheim¹, Stefano Di Talia, Eric D Siggia

¹Center for Studies in Physics and Biology, The Rockefeller University, New York 10065, USA. skotheim@stanford.edu

Nature

|July 18, 2008

まとめ

芽生える酵母酵母

科学分野:

細胞生物学細胞生物学
分子生物学は分子生物学である.
システム生物学システム生物学

背景:

Saccharomyces cerevisiaeのスタートチェックポイントは,細胞サイクルへの入り口のシグナルを統合しています.
現存するモデルでは,スタートのアクティベーションの線形カスケードが提案されています.

研究の目的:

芽生える酵母におけるスタートチェックポイントの規制ネットワークを調査する.
細胞周期調節におけるフィードバックメカニズムの役割を明らかにする.

主な方法:

遺伝子消去変異体 (CLN1およびCLN2) の分析.
G1/S regulon.の転写調節に関する調査.
芽生えの効率と細胞サイクル進行の評価.

主要な成果:

スタートは,システムレベルのフィードバックにより,スイッチとして機能します.
G1サイクリン (Cln1とCln2) の転写的陽性フィードバックは,G1/Sレギロン発現を迅速に誘導する.
核Cln2は遺伝子発現を調節し,細胞質Cln2は芽生えを促進する.

結論:

ポジティブなフィードバックは,Cln1とCln2の発現を増幅し,堅固な芽生えと一貫したG1/Sレギュロン活性化を保証します.
哺乳類の細胞における同様の規制構造は,保存されたメカニズムまたは収束進化を示唆する.

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