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関連する概念動画

DNA Damage can Stall the Cell Cycle02:36

DNA Damage can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
DNA Damage Can Stall the Cell Cycle02:36

DNA Damage Can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
Anaphase Promoting Complex00:50

Anaphase Promoting Complex

The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...
Anaphase Promoting Complex00:50

Anaphase Promoting Complex

The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...
Restarting Stalled Replication Forks02:37

Restarting Stalled Replication Forks

DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart, a...
Separation of Sister Chromatids02:17

Separation of Sister Chromatids

At the transition from prophase to metaphase, there is a reduction in cohesion along the chromosomal arms, resulting in the resolution of sister chromatids. However, residual cohesin connections remain to hold the sister chromatids together until the transition from metaphase to anaphase. The residual connection prevents any premature separation of sister chromatids, blocking the risks of aneuploidy within the daughter cells.
At the onset of anaphase, separase, a proteolytic enzyme, is...

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関連する実験動画

Updated: Jul 3, 2026

Two- and Three-Dimensional Live Cell Imaging of DNA Damage Response Proteins
10:24

Two- and Three-Dimensional Live Cell Imaging of DNA Damage Response Proteins

Published on: September 28, 2012

Cdc14BとAPC/Cは,DNAの損傷に対処しています.

Peter De Wulf1, Rosella Visintin

  • 1Department of Experimental Oncology, European Institute of Oncology, IFOM-IEO Campus, Via Adamello 16, 20139 Milan, Italy.

Cell
|July 30, 2008
PubMed
まとめ

芽生えた酵母細胞サイクルレギュレータCdc14,APC/C(Cdh1),Plk1は,ヒト細胞で再接続されています. このネットワークは,DNA損傷に対する反応として,細胞サイクルを制御します.

科学分野:

  • 細胞生物学 細胞生物学
  • 分子生物学は分子生物学である.
  • 遺伝学 遺伝学とは

背景:

  • 芽生えた酵母におけるミトスの出口は,細胞循環の重要なプロセスである.
  • 重要な規制者はCdc14,APC/C ((Cdh1),Plk1.1) を含む.
  • これらのレギュレータを理解することは,細胞サイクル制御に不可欠です.

研究 の 目的:

  • 人間の細胞における芽生えた酵母菌のミトーシス脱出レギュレータの役割を調査する.
  • ヒトの細胞サイクル制御において,タンパク質ネットワークが保存されるか,再配線されるかを判断する.
  • ヒト細胞のDNA損傷に対するこのネットワークの反応を探求する.

主な方法:

  • イーストとヒトの細胞におけるタンパク質ネットワークの比較分析.
  • 人間の細胞サイクルにおけるCdc14,APC/C(Cdh1),Plk1の機能を調査する.
  • DNA損傷誘導後の細胞サイクル進行を評価する.

主要な成果:

  • Cdc14,APC/C(Cdh1),Plk1のネットワークは,ヒト細胞で再接続されている.
  • この再配線されたネットワークは,DNA損傷に対する反応として,細胞サイクル進行を制御する.

さらに関連する動画

Visualization of DNA Repair Proteins Interaction by Immunofluorescence
07:55

Visualization of DNA Repair Proteins Interaction by Immunofluorescence

Published on: June 26, 2020

Detection and Visualization of DNA Damage-induced Protein Complexes in Suspension Cell Cultures Using the Proximity Ligation Assay
13:10

Detection and Visualization of DNA Damage-induced Protein Complexes in Suspension Cell Cultures Using the Proximity Ligation Assay

Published on: June 9, 2017

関連する実験動画

Last Updated: Jul 3, 2026

Two- and Three-Dimensional Live Cell Imaging of DNA Damage Response Proteins
10:24

Two- and Three-Dimensional Live Cell Imaging of DNA Damage Response Proteins

Published on: September 28, 2012

Visualization of DNA Repair Proteins Interaction by Immunofluorescence
07:55

Visualization of DNA Repair Proteins Interaction by Immunofluorescence

Published on: June 26, 2020

Detection and Visualization of DNA Damage-induced Protein Complexes in Suspension Cell Cultures Using the Proximity Ligation Assay
13:10

Detection and Visualization of DNA Damage-induced Protein Complexes in Suspension Cell Cultures Using the Proximity Ligation Assay

Published on: June 9, 2017

  • 細胞循環調節のための保存されながら適応されたメカニズムを示しています.

    • 結論:

    • Cdc14,APC/C(Cdh1),Plk1を含む細胞サイクル制御ネットワークは,適応可能である.
    • 人間の細胞は,DNA損傷反応のために,このネットワークの改造されたバージョンを使用します.
    • この発見は,基本的な生物学的プロセスにおける進化的可塑性を強調しています.