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Updated: Jul 3, 2026

Using Modified Synthetic Oligonucleotides to Assay Nucleic Acid-Metabolizing Enzymes
05:33

Using Modified Synthetic Oligonucleotides to Assay Nucleic Acid-Metabolizing Enzymes

Published on: July 5, 2024

インジケーターシフトアッセイは分子タイマーとして動作します.

Andrey Buryak1, Friederike Zaubitzer, Alexei Pozdnoukhov

  • 1Institut des Sciences et Ingénierie Chimiques, Ecole Polytechnique Fédérale de Lausanne (EPFL), Switzerland.

Journal of the American Chemical Society
|August 6, 2008
PubMed
まとめ

ダイナミックなRh染料複合体は,化学反応を追跡するための新しい方法を提供します. これらの複合体による紫外線スペクトロスコピーは,アデノシン三リン酸 (ATP) やアデノシン二リン酸 (ADP) の添加などの事象の歴史を明らかにすることができます.

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Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...