酵母S. cerevisiaeにおけるプロフィリンとCAPの間の機能的リンクの証拠

A Vojtek¹, B Haarer, J Field

¹Cold Spring Harbor Laboratory, New York 11724-2208.

Cell

|August 9, 1991

まとめ

CAPと酵母プロフィリン (PFY) は,成長信号と細胞骨格の改造を結びつけています. CAPの損失について

科学分野:

細胞生物学細胞生物学
分子生物学は分子生物学である.
バイオケミストリーバイオケミストリー

背景:

CAPは,Saccharomyces cerevisiaeアデニリルサイクラゼ複合体の構成要素である.
CAPのN端領域は,細胞のRAS反応を媒介する.
C末端のCAP領域の喪失は,形態学的および栄養学的欠陥を引き起こす.

研究の目的:

CAP C端末ドメインの機能を調査する.
酵母におけるCAPとプロフィリン (PFY) の関係を解明する.
細胞骨格の調節を支える分子機構を理解する.

主な方法:

カップ酵母菌の変異体のフェノタイプ分析.
PFYの過剰発現を含む遺伝子解析.
変異酵母とアカンタモエバプロフィンを用いた生化学研究.

主要な成果:

cap-細胞はランダムな芽生えと不適切なアクチン分布を示します.
PFYの過剰発現は,キャップ細胞の欠陥を抑制する.
プロフィリンがキャップ・フェノタイプを抑制する能力は,アクチン結合のみではなく,ポリフォスフォニノシチド結合に依存しています.

結論:

CAPとプロフィリンは,成長信号を酵母体内の細胞骨格の改造と結びつけるのに不可欠です.
プロフィリンのポリフォスフォノシチド結合能力は,CAP媒介のプロセスにおけるプロフィリンの役割にとって不可欠です.
この研究は,細胞組織におけるCAPとプロフィリンを含む新しいシグナル伝達経路を明らかにしています.

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