細菌のエフェクタータンパク質によるマイクロRNA経路の抑制
Lionel Navarro1, Florence Jay, Kinya Nomura
1Institut de Biologie Moléculaire des Plantes, CNRS UPR 2353-Université Louis Pasteur, 12 Rue du Général Zimmer, 67084 Strasbourg Cedex, France.
まとめ
マイクロRNA (miRNA) は,細菌病原体に対する植物防御に不可欠です. miRNAsが欠けている植物は,細菌感染に対する抵抗性を高め,miRNAsが基礎免疫を抑制することを示しています.
科学分野:
- 植物免疫 植物免疫とは
- マイクロRNA (miRNA) 調節規制について
- バクテリアの病原化による病原化.
背景:
- 植物や動物は,病原体に関連した分子パターン (PAMP) を検出し,防御反応を誘発する.
- マイクロRNA (miRNA) は,PAMP知覚によって差異的に調節されるが,先天性免疫におけるその役割は不明である.
研究 の 目的:
- 生まれつきの免疫を植えるためのmiRNA経路の貢献を調査する.
- miRNA経路を標的とする細菌エフェクターを特定する.
主な方法:
- アラビドプシスのmiRNA欠乏変異体の分析.
- 野生型および変異植物におけるPseudomonas syringaeおよびその他の菌株の細菌増殖を評価する.
- ミRNA機能抑制に関与するPseudomonas syringaeエフェクターを特定する.
主要な成果:
- アラビドプシスのミRNAが欠乏した変異体は,分泌欠陥のあるタイプIIIの変異体である Pseudomonas syringae の部分的に回復した成長を示した.
- これらのmiRNA欠乏型変異体は,非病原性Pseudomonas fluorescensとEscherichia coliの成長にも貢献した.
- PAMP反応性miRNAの活性化,生殖,安定性,または活動を抑制するPseudomonas syringaeエフェクターが特定されました.
結論:
- マイクロRNAは,細菌病原体に対する植物基礎防御の重要な構成要素である.
- バクテリアは,ウイルスと同様に,感染を成功させるためのRNAサイレンシング経路を抑制するメカニズムを進化させた.
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