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Introduction to Nuclear Reprogramming

Introduction to Nuclear Reprogramming

Nuclear reprogramming is the process of switching gene expression of one cell type to that of another cell type, usually from a differentiated cell state to an undifferentiated cell state. Differentiation occurs during processes such as development and morphogenesis, tissue regeneration, and malignancy. Cells can also be artificially induced to reprogram their gene expression by techniques such as nuclear transfer, induced pluripotency, and cell fusion. Such techniques have many applications in...

Methods of Nuclear Reprogramming

Methods of Nuclear Reprogramming

Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for injury repair.

Somatic to iPS Cell Reprogramming

Somatic to iPS Cell Reprogramming

Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012 for this...

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Isolation of Viral Replication Compartment-enriched Sub-nuclear Fractions from Adenovirus-infected Normal Human Cells

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Published on: November 12, 2015

アデノウイルス e1aによる表皮遺伝的再プログラム.

Roberto Ferrari¹, Matteo Pellegrini, Gregory A Horwitz

¹Department of Biological Chemistry, University of California, Los Angeles, CA 90095, USA.

Science (New York, N.Y.)

|August 23, 2008

まとめ

この要約は機械生成です。

アデノウイルス e1aのタンパク質はヒト細胞を再プログラムし,ヒストンのアセチル化を変化させ,抗ウイルス反応を抑制します. この表遺伝的再プログラミングは,細胞循環を刺激し,細胞の変容を促進します.

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Adenovirus-mediated Genetic Removal of Signaling Molecules in Cultured Primary Mouse Embryonic Fibroblasts

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Last Updated: Jul 2, 2026

Isolation of Viral Replication Compartment-enriched Sub-nuclear Fractions from Adenovirus-infected Normal Human Cells

Isolation of Viral Replication Compartment-enriched Sub-nuclear Fractions from Adenovirus-infected Normal Human Cells

Published on: November 12, 2015

Combined Genetic and Chemical Capsid Modifications of Adenovirus-Based Gene Transfer Vectors for Shielding and Targeting

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Published on: October 26, 2018

Adenovirus-mediated Genetic Removal of Signaling Molecules in Cultured Primary Mouse Embryonic Fibroblasts

Adenovirus-mediated Genetic Removal of Signaling Molecules in Cultured Primary Mouse Embryonic Fibroblasts

Published on: September 9, 2010

科学分野:

エピジェネティクスエピジェネティクス
分子生物学は分子生物学である.
ウイルス学ウイルス学ウイルス学

背景:

アデノウイルス e1aのタンパク質は,静止状態のヒト細胞の細胞増殖を誘発することが知られている.
e1aがこの細胞再プログラムを達成する正確な分子機構は,完全に理解されていません.

研究の目的:

ヒト細胞の表遺伝子再プログラムにおけるアデノウイルスe1aの役割を調査する.
重要な調節タンパク質とe1aの相互作用の時間的動態とその遺伝子発現への影響を解明する.

主な方法:

クロマチン免疫プレシピテーション (ChIP) により,遺伝子プロモーターにおけるタンパク質結合とヒストンの改変を分析する.
タンパク質レベルとヒストンのアセチル化を評価するためのウェスタン・ブロッティング.
転写変化を決定するための遺伝子発現分析.

主要な成果:

アデノウイルスe1aは,網膜芽細胞腫 (RB) タンパク質とp300/CBPヒストンアセチルトランスフェラーゼのグローバル再配置を引き起こします.
e1aはヒストン3リジン-18アセチル化 (H3K18ac) を限られた遺伝子セットに制限し,細胞サイクルを刺激し,抗ウイルス反応を抑制します.
e1aが細胞サイクル,抗ウイルス,分化遺伝子のプロモーターに一時的に結合すると,転写の変化が調節されます.

結論:

アデノウイルスe1aは,定義された表遺伝的再プログラミングプロセスを通して細胞の変容を促します.
e1aがp300/CBPおよびRBタンパク質と一時的に相互作用することは,その機能にとって極めて重要です.
これらのメカニズムを理解することで,ウイルス腫瘍形成と細胞変異の洞察が得られます.