反対のWnt経路は,オルガノゲネシス中に細胞の極性を指向します
Jennifer L Green1, Takao Inoue, Paul W Sternberg
1Howard Hughes Medical Institute/Division of Biology, California Institute of Technology, Mail Code 156-29, Pasadena, CA 91125, USA.
Cell
|August 30, 2008
まとめ
組織の極性は,複数のWnt経路を統合することに依存しています. C. elegans vulvaの非対称な細胞分裂は,細胞を方向づけ,新しいシグナリングメカニズムを通じて基底の極性を確立するためにWntsを使用します.
科学分野:
- 発達生物学 発達生物学について
- 細胞生物学 細胞生物学
- 遺伝学 遺伝学とは
背景:
- 非対称な細胞分裂は,組織と臓器の組織にとって極めて重要です.
- C. elegans vulvaの発達において,鏡像対称性は,vulva前駆細胞 (VPC) の対極的な分裂方向性によって達成される.
研究 の 目的:
- C. elegans.におけるVPCの非対称的な分裂パターンを支配する分子メカニズムを特徴づける.
- 複数のWntシグナル伝達経路が組織の極性形成にどのように貢献するか解明する.
主な方法:
- Wnt信号構成要素 (MOM-2, LIN-44, EGL-20) とその受容体 (Fz/LIN-17, Ryk/LIN-18, CAM-1, VANG-1) の分析について.
- ベータ-カテニンの局所化と遺伝子転写の活性化を調査する.
- 細胞の"地面"の極性の確立を研究する.
主要な成果:
- シンメトリー軸で表されるMOM-2とLIN-44のWntsは,Fz/LIN-17とRyk/LIN-18経路を中心にVPCを方向づけます.
- 均一な"地面"の極性は,Wnt/EGL-20シグナリングによって確立されています.
- EGL-20は,Ror受容体チロシンキナーゼCAM-1と平面細胞の極性成分であるVan Gogh/VANG-1を含む新しい経路を使用しています.
結論:
- 組織の極性は,異なるWnt信号伝達経路の統合によって決定されます.
- 複数のWnt経路が協力して,細胞分裂の方向性を制御し, vulva の発達中に極性を確立します.
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