核分裂酵母におけるプロモーター駆動スプライシング制御
Alberto Moldón1, Jordi Malapeira, Natalia Gabrielli
1Oxidative Stress and Cell Cycle Group, Universitat Pompeu Fabra, C/Doctor Aiguader 88, Barcelona 08003, Spain.
Nature
|September 26, 2008
まとめ
分裂酵母におけるメオシス特有のサイクリンであるRem1は,転写とスプライシングによって調節されます. Mei4とFkh2は,その発現を制御し,中間再結合と細胞サイクル進行に影響を与えます.
科学分野:
- 分子生物学は分子生物学である.
- 細胞サイクル規制について
- イースト遺伝学 イースト遺伝学
背景:
- メイオシスはミトシスとは異なるが,メイオシス前S相,二つの分裂,そしてユニークな染色体分離がある.
- Rem1は,再結合と間に合ったメオシスIの発生に不可欠な *Schizosaccharomyces pombe* のメオシス固有のサイクリンです.
- エクトピックRem1発現は,植物性細胞でG1停止とミトスの破滅を引き起こします.
研究 の 目的:
- 転写および転写後のレベルでの *rem1* 遺伝子発現の規制メカニズムを調査する.
- 特定の転写因子の *rem1* スプライシングと機能を制御する役割を解明する.
主な方法:
- 転写とスプライシングによる *rem1* 遺伝子調節の分析.
- *rem1*プロモーターが他のイントロンを含む遺伝子と融合したキメリック遺伝子の構築と分析.
- フォークヘッドの転写因子Mei4とFkh2.2の関与を調査した.
主要な成果:
- *rem1*発現は,転写と代替スプライシングの両方で制御され,異なるタンパク質を生成します.
- *rem1* のスプレッシング規制は,その転写された領域とは独立しています.
- Mei4は*rem1*の転写とスプライシングを促進し,Fkh2は植物性成長とS段階のプレミオティック期におけるイントロン保持を媒介する.
結論:
- *rem1* splicingは meiotic-specificであり,Mei4とFkh2.2によって規制されている.
- Fkh2は*rem1*スプライシングを調節し,特定の細胞サイクル段階でイントロンの保持を促進する二重の役割を果たします.
- これらの発見は,S. pombe*における中性進行に不可欠な新しい遺伝子調節層を明らかにしています.
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