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Induced Pluripotent Stem Cells01:06

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic cells are...
Induced Pluripotent Stem Cells01:13

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore called induced pluripotent stem...
Induced Pluripotent Stem Cells01:13

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore called induced pluripotent stem...
EPS and iPS Cells in Disease Research01:21

EPS and iPS Cells in Disease Research

Embryonic and induced pluripotent stem cells are excellent models for disease research because of their ability to self-renew and differentiate into most cell types. Somatic cells from a patient are isolated and reprogrammed into induced pluripotent stem cells or iPSCs. These iPSCs are later differentiated into the desired cell type, which mirrors the diseased cell of the patient. In this way, disease models have been created for investigating diseases such as Down syndrome, type I diabetes,...
iPS Cell Differentiation01:22

iPS Cell Differentiation

The ability of induced pluripotent stem cells or iPSCs to differentiate into most body cell types has stimulated repair and regenerative medicine research over the past few decades. iPSC-derived blood cells, hepatocytes, beta islet cells, cardiomyocytes, neurons, and other cell types can repair injuries or regenerate damaged tissue in diseases such as diabetes and neurodegenerative disorders.
Somatic to iPS Cell Reprogramming01:29

Somatic to iPS Cell Reprogramming

Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012 for this...

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関連する実験動画

Updated: Jun 30, 2026

Efficient Generation Human Induced Pluripotent Stem Cells from Human Somatic Cells with Sendai-virus
09:43

Efficient Generation Human Induced Pluripotent Stem Cells from Human Somatic Cells with Sendai-virus

Published on: April 23, 2014

誘発性多能性幹細胞は,ウイルスの統合なしに生成されます.

Matthias Stadtfeld1, Masaki Nagaya, Jochen Utikal

  • 1Massachusetts General Hospital Cancer Center and Center for Regenerative Medicine, 185 Cambridge Street, Boston, MA 02114, USA.

Science (New York, N.Y.)
|September 27, 2008
PubMed
まとめ
この要約は機械生成です。

研究者はマウス誘発型多能幹細胞 (iPS) を非統合性アデノウイルスを用いて作成し,有害なゲノム統合性ウイルスを避けました. この安全性の高い方法は,細胞の再プログラムに成功するために遺伝的統合が不要であることを示しています.

さらに関連する動画

Generation of Induced Pluripotent Stem Cells from Frozen Buffy Coats using Non-integrating Episomal Plasmids
10:52

Generation of Induced Pluripotent Stem Cells from Frozen Buffy Coats using Non-integrating Episomal Plasmids

Published on: June 5, 2015

Generation of Integration-free Induced Pluripotent Stem Cells from Human Peripheral Blood Mononuclear Cells Using Episomal Vectors
09:45

Generation of Integration-free Induced Pluripotent Stem Cells from Human Peripheral Blood Mononuclear Cells Using Episomal Vectors

Published on: January 1, 2017

関連する実験動画

Last Updated: Jun 30, 2026

Efficient Generation Human Induced Pluripotent Stem Cells from Human Somatic Cells with Sendai-virus
09:43

Efficient Generation Human Induced Pluripotent Stem Cells from Human Somatic Cells with Sendai-virus

Published on: April 23, 2014

Generation of Induced Pluripotent Stem Cells from Frozen Buffy Coats using Non-integrating Episomal Plasmids
10:52

Generation of Induced Pluripotent Stem Cells from Frozen Buffy Coats using Non-integrating Episomal Plasmids

Published on: June 5, 2015

Generation of Integration-free Induced Pluripotent Stem Cells from Human Peripheral Blood Mononuclear Cells Using Episomal Vectors
09:45

Generation of Integration-free Induced Pluripotent Stem Cells from Human Peripheral Blood Mononuclear Cells Using Episomal Vectors

Published on: January 1, 2017

科学分野:

  • 幹細胞生物学 幹細胞生物学とは
  • 分子生物学は分子生物学である.
  • 遺伝学 遺伝学とは

背景:

  • 多能幹細胞は再生医療において極めて重要です.
  • 誘発性多能幹細胞 (iPS) の生成のための現在の方法は,しばしばウイルスを統合することに依存し,安全性に関する懸念を提起しています.
  • Oct4,Sox2,Klf4,c-Mycは,細胞の再プログラムのための重要な転写因子である.

研究 の 目的:

  • 誘発性多能幹細胞 (iPS) を生成するためのより安全な方法を開発する.
  • 非統合性ウイルスベクトルが,体細胞を効率的に再プログラムできるかどうかを調査する.
  • ウイルスによって再プログラムされた細胞の多能性と発達可能性を評価する.

主な方法:

  • フィブロブラストと肝臓細胞からマウスのiPS細胞を生成する.
  • 非統合性アデノウイルスを使用したOct4,Sox2,Klf4,c-Mycの一時的な発現.
  • DNA脱メチル化,多能性遺伝子発現,テラトマ形成,キメリックマウスにおける生殖系貢献の分析.

主要な成果:

  • 移行性非統合性アデノウイルス発現を用いたマウスアデノウイルス iPS (アデノ-iPS) 細胞を成功裏に生成した.
  • アデノ-iPS細胞は特徴的なDNA脱メチル化を示し,内在的な多能性マーカーを発現した.
  • これらの細胞はテラトーマを形成し,キメリックマウスの生殖系統を含む様々な組織に寄与した.

結論:

  • 挿入性ミュータゲネシスは,体細胞の体外再プログラム化のための前提条件ではありません.
  • アデノウイルスの再プログラミングは,iPS細胞を生成するためのより安全な代替案を提供します.
  • この改良された方法は,患者特有の幹細胞の研究や,胚性幹細胞とiPS細胞の比較を容易にする.