トウモロコシのViviparous-1発達遺伝子は,新しい転写活性化剤をコードする
D R McCarty1, T Hattori, C B Carson
1Vegetable Crops Department, University of Florida, Gainesville 32611.
Cell
|September 6, 1991
まとめ
トウモロコシのViviparous-1 (Vp1) 遺伝子は,種子の成熟に不可欠な新しい転写因子をコードします. このタンパク質は遺伝子発現を活性化し,種子発達の過程で植物ホルモン反応の役割を示唆しています.
科学分野:
- 植物分子生物学 植物分子生物学
- 発達生物学 発達生物学とは
- 遺伝学 遺伝学とは
背景:
- トウモロコシのViviparous-1 (Vp1) 遺伝子は,種子の成熟を調節する上で極めて重要です.
- Vp1の分子機構を理解することは,種子の発達経路を解読する鍵です.
研究 の 目的:
- Vp1遺伝子産物の特徴と,トウモロコシの種子発育におけるその機能を明らかにする.
- 転写活性化に責任を負うVp1タンパク質の機能ドメインを特定する.
主な方法:
- Vp1遺伝子クローニングとタンパク質の特徴化.
- 活性化配列を特定するために,Vp1を GAL4 DNA結合ドメインに融合させる.
- トランザクティベーションの可能性を評価するために,トウモロコシ原生体におけるレポーター遺伝子解析.
主要な成果:
- Vp1は73,335ダルトンのタンパク質をコードし,既知のタンパク質に同質性が欠けている.
- Vp1.1内に酸性転写活性化ドメインが特定されました.
- Vp1は,トウモロコシ原生体におけるレポーター遺伝子発現を130倍以上強く活性化させた.
- 酸性ドメインはトランザクティベーションに不可欠であり,VP16アクティベーター配列によって機能的に置き換えることができる.
結論:
- Vp1は,トウモロコシにおける新しい転写因子として機能する.
- 特定された酸性ドメインは,Vp1のトランザクティベーション活動に不可欠です.
- Vp1は,発達の過程で種子特有のホルモン反応を強化する可能性があります.
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