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調節因子によるクラスII遺伝子転写の活性化は,新しい活性によって強化されます
M Meisterernst1, A L Roy, H M Lieu
1Laboratory of Biochemistry and Molecular Biology, Rockefeller University, New York, New York 10021.
Cell
|September 6, 1991
まとめ
新しい活性化剤依存型転写因子USAが特定されました. TFIIDと相互作用することによって転写を調節し,アクティベーター機能を強化する2つの成分で構成されています.
科学分野:
- 分子生物学は分子生物学である.
- バイオケミストリー バイオケミストリー
- 遺伝子規制 遺伝子規制
背景:
- 転写活性化剤は,基底転写機構と相互作用することで,遺伝子発現を調節する.
- TFIID複合体は,転写の開始において重要な役割を果たします.
研究 の 目的:
- アクティベータ依存転写を刺激する新しい活性 (米国) を特徴づける.
- 米国が転写開始に影響を与えるメカニズムを解明する.
主な方法:
- 浄化されたコンポーネントを使用して再構成された転写システム.
- ゲルモビリティシフトアッセイは,タンパク質-DNAおよびタンパク質-タンパク質の相互作用を研究するためのものです.
- 新しい活動の浄化 (米国).
主要な成果:
- 米国は,自然TFIID.と再構成されたシステムで,10〜50倍の活性化剤依存トランスクリプションを刺激しました.
- USAは,2つのコンポーネントに分解され,コアプロモーター活動に相反する効果を持つアクティベーター機能を強化した.
- ゲル移動シフト実験では,USAとTFIIDの阻害成分との物理的な相互作用が明らかになった.
結論:
- USAには,プレイニシテーション複合体と関連するコファクターが含まれています.
- これらのコファクターは基礎プロモーターの活性を低下させ,活性化剤依存型転写を強化する.
- 米国は,転写開始における新しい規制メカニズムを表しています.
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