関連する実験動画
Updated: Jun 28, 2026
08:55
Expression, Solubilization, and Purification of Eukaryotic Borate Transporters
Published on: March 7, 2019
核塩基-カチオン-シンポート-1トランスポーターファミリーの構造と分子機構
Simone Weyand1, Tatsuro Shimamura, Shunsuke Yajima
1Membrane Protein Laboratory, Diamond Light Source Limited, Harwell Science and Innovation Campus, Chilton, Didcot, Oxfordshire OX11 0DE, UK.
Science (New York, N.Y.)
|October 18, 2008
まとめ
核塩基-カタニン-シンポート-1 (NCS1) トランスポーターMHP1は,
科学分野:
- 構造生物学 構造生物学とは
- メンブレーン輸送 メンブレーン輸送
- バイオケミストリー バイオケミストリー
背景:
- 核塩基-カチオン-シンポート-1 (NCS1) トランスポーターは,核塩基を救出するために不可欠です.
- 構造と機能の関係を理解することは,細胞の代謝物輸送を解読する鍵です.
研究 の 目的:
- Mhp1トランスポーターの高解像度構造を決定する.
- NCS1トランスポーターにおける基板結合と輸送のメカニズムを解明する.
主な方法:
- 2.85-アングストロムの解像度のX線結晶学.
- Mhp1.1の外向きの開いた形状と基板に縛られた閉じた形状の決定.
主要な成果:
- ベンジルヒダントオイントランスポーターであるMhp1の2.85アングストロムの構造は解明されました.
- Mhp1は12のトランスメブランヘリクで構成され,10の反転繰り返しがあります.
- 構造の変化は,外向きの穴が基板結合時にどのように閉まるかを明らかにします.
結論:
- Mhp1の構造は,NCS1トランスポーターの交代アクセスメカニズムについての洞察を提供します.
- 腔の対称的な配置と同期したヘリックス運動は,輸送ダイナミクスを説明します.
- この研究は,関連する膜輸送タンパク質を理解するための基礎を築く.
関連する概念動画
01:31ABC Transporters: Exporter
ATP-binding cassette or ABC transporter is the largest superfamily of integral membrane proteins. The transporters have transmembrane-binding domains (TMDs) and nucleotide-binding domains (NBDs). The TMDs are specific to their substrates, whereas the NBDs are similar to engines that complete ATP hydrolysis to complete the substrate transport. They can be full transporters consisting of two TMDs and NBDs, half transporters with one TMD and NBD, while some encoded with a single TMD or NBD are...
01:31Membrane Transporters
Transporters are essential membrane transport proteins with functions related to cell nutrition, homeostasis, communication, etc. Approximately 7% of all genes in the human genome code for transporters or transporter-related proteins.
Transporters are mainly composed of alpha-helices, built from bundles of ten or more helices traversing the plasma membrane. The solute-binding sites are located midway, where some of the helices are broken or distorted, making space for the binding site through...
Transporters are mainly composed of alpha-helices, built from bundles of ten or more helices traversing the plasma membrane. The solute-binding sites are located midway, where some of the helices are broken or distorted, making space for the binding site through...
01:14Active Transport
Active transport is a critical biological process that allows cells to move solutes against an electrochemical gradient. This process requires direct energy input and is characterized by its selectivity, saturability, and susceptibility to competitive inhibition.
Primary active transporters, like Na+, K+ and -ATPase, directly utilize ATP to move ions across the membrane. These transporters play significant roles in various physiological processes. For instance, Na+, K+ and -ATPase maintain...
Primary active transporters, like Na+, K+ and -ATPase, directly utilize ATP to move ions across the membrane. These transporters play significant roles in various physiological processes. For instance, Na+, K+ and -ATPase maintain...
01:32Secondary Active Transport
One example of how cells use the energy contained in electrochemical gradients is demonstrated by glucose transport into cells. The ion vital to this process is sodium (Na+), which is typically present in higher concentrations extracellularly than in the cytosol. Such a concentration difference is due, in part, to the action of an enzyme "pump" embedded in the cellular membrane that actively expels Na+ from a cell. Importantly, as this pump contributes to the high concentration of...
01:55Secondary Active Transport
One example of how cells use the energy contained in electrochemical gradients is demonstrated by glucose transport into cells. The ion vital to this process is sodium (Na+), which is typically present in higher concentrations extracellularly than in the cytosol. Such a concentration difference is due, in part, to the action of an enzyme “pump” embedded in the cellular membrane that actively expels Na+ from a cell. Importantly, as this pump contributes to the high concentration of...
01:32Secondary Active Transport
One example of how cells use the energy contained in electrochemical gradients is demonstrated by glucose transport into cells. The ion vital to this process is sodium (Na+), which is typically present in higher concentrations extracellularly than in the cytosol. Such a concentration difference is due, in part, to the action of an enzyme "pump" embedded in the cellular membrane that actively expels Na+ from a cell. Importantly, as this pump contributes to the high concentration of...