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Updated: Jun 28, 2026

Dissolution Dynamic Nuclear Polarization Instrumentation for Real-time Enzymatic Reaction Rate Measurements by NMR
10:54

Dissolution Dynamic Nuclear Polarization Instrumentation for Real-time Enzymatic Reaction Rate Measurements by NMR

Published on: February 23, 2016

In situ 温度のジャンプダイナミックな核極化:溶液中の二次元13C-13C相関スペクトロスコピーの感度向上

Chan-Gyu Joo1, Andrew Casey, Christopher J Turner

  • 1Francis Bitter Magnet Laboratory and Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.

Journal of the American Chemical Society
|October 24, 2008
PubMed
まとめ

ダイナミックな核極化と温度ジャンプの組み合わせにより,2D 13C-13C NMRの感度が100~170倍に劇的に向上します. この新しいアプローチは,スピン極化を改善するためにレーザー誘発溶融を使用しています.

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Real-Time Metabolic Detection in Living Cells Using Hyperpolarized 13C NMR

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Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy14:55

Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy

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¹³C NMR: ¹H–¹³C Decoupling

The probability of having two carbon-13 atoms next to each other is negligible because of the low natural abundance of carbon-13. Consequently, peak splitting due to carbon-carbon spin-spin coupling is not observed in spectra. However, protons up to three sigma bonds away split the carbon signal according to the n+1 rule, resulting in complicated spectra.
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2D NMR: Overview of Homonuclear Correlation Techniques

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COSY90 is the standard two-dimensional (2D) COSY experiment that...
2D NMR: Overview of Heteronuclear Correlation Techniques01:18

2D NMR: Overview of Heteronuclear Correlation Techniques

Heteronuclear correlation spectroscopy is an analytical technique that investigates the coupling between different types of nuclei, often a proton and an X-nucleus, such as carbon-13 or nitrogen-15. This method is commonly used in nuclear magnetic resonance (NMR) spectroscopy to gain insights into complex chemical compounds' structural and compositional aspects. A typical heteronuclear correlation spectrum displays X-nucleus chemical shifts on one axis and a proton spectrum on the other axis.
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Insensitive Nuclei Enhanced by Polarization Transfer (INEPT)

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¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)

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NMR Spectroscopy: Spin–Spin Coupling

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