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関連する概念動画

Circadian Rhythms and Gene Regulation02:19

Circadian Rhythms and Gene Regulation

The biological clock is involved in many aspects of regulating complex physiology in all animals. It was in 1935 when German zoologists, Hans Kalmus and Erwin Bünning, discovered the existence of circadian rhythm in Drosophila melanogaster. However, the internal molecular mechanisms behind the circadian clock remained a mystery until 1984, when Jeffrey C. Hall, Michael Rosbash, and Michael W. Young discovered the expression of the Per gene oscillating over a 24-hour cycle. In subsequent years,...
Circadian Rhythms and Gene Regulation02:19

Circadian Rhythms and Gene Regulation

The biological clock is involved in many aspects of regulating complex physiology in all animals. It was in 1935 when German zoologists, Hans Kalmus and Erwin Bünning, discovered the existence of circadian rhythm in Drosophila melanogaster. However, the internal molecular mechanisms behind the circadian clock remained a mystery until 1984, when Jeffrey C. Hall, Michael Rosbash, and Michael W. Young discovered the expression of the Per gene oscillating over a 24-hour cycle. In subsequent years,...
Biological Clocks and Seasonal Responses02:45

Biological Clocks and Seasonal Responses

The circadian—or biological—clock is an intrinsic, timekeeping, molecular mechanism that allows plants to coordinate physiological activities over 24-hour cycles called circadian rhythms. Photoperiodism is a collective term for the biological responses of plants to variations in the relative lengths of dark and light periods. The period of light-exposure is called the photoperiod.
Chronopharmacokinetics: Circadian Rhythms and Influence on Drug Response01:15

Chronopharmacokinetics: Circadian Rhythms and Influence on Drug Response

Circadian rhythms are cyclic changes that are crucial in plasma drug concentrations. Various standard circadian parameters, including core body temperature, heart rate, and other cardiovascular factors, directly impact disease states and the therapeutic response to drug therapy.
The time of drug administration is an important factor to consider, as it can influence the toxic dose of a drug. For example, a study conducted by Prins et al. in 1997 examined the effects of the timing of...
Operon Model01:23

Operon Model

The operon model represents a fundamental mechanism of gene regulation in prokaryotes, enabling coordinated expression of genes involved in related metabolic or functional pathways. Operons consist of structural genes, a promoter, and an operator, with transcription regulated by repressors, activators, and small effector molecules.Structure and Function of OperonsAn operon is a cluster of structural genes transcribed together under the control of a single promoter. The promoter region...
RLC Circuit as a Damped Oscillator01:30

RLC Circuit as a Damped Oscillator

An RLC circuit combines a resistor, inductor, and capacitor, connected in a series or parallel combination.
Consider a series RLC circuit. Here, the presence of resistance in the circuit leads to energy loss due to joule heating in the resistance. Therefore, the total electromagnetic energy in the circuit is no longer constant and decreases with time. Since the magnitude of charge, current, and potential difference continuously decreases, their oscillations are said to be damped. This is...

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関連する実験動画

Updated: Jun 28, 2026

Parallel Measurement of Circadian Clock Gene Expression and Hormone Secretion in Human Primary Cell Cultures
06:53

Parallel Measurement of Circadian Clock Gene Expression and Hormone Secretion in Human Primary Cell Cultures

Published on: November 11, 2016

シルカディアンオシレータの構造に関する洞察

Carl Hirschie Johnson1, Martin Egli, Phoebe L Stewart

  • 1Department of Biological Sciences, Box 35-1634, Vanderbilt University, Nashville, TN 37235-1634, USA. carl.h.johnson@vanderbilt.edu

Science (New York, N.Y.)
|November 1, 2008
PubMed
まとめ

サイアノバクテリアは,日々の細胞制御のために3つのタンパク質の昼夜振動器 (KaiA,KaiB,KaiC) を利用します. 構造と生化学の研究は,生物学的タイムキープに不可欠な,そのラッチェッティングメカニズムを明らかにします.

科学分野:

  • 微生物学 微生物学とは
  • バイオケミストリー バイオケミストリー
  • 構造生物学 構造生物学とは

背景:

  • サイアノバクテリアは,遺伝子発現のような細胞過程を調節する内生的な昼夜システムを持っています.
  • 染色体トポロジーと圧縮の毎日のサイクルは,サイアノバクテリアで観察されています.
  • シルカディアンリズムとは,様々な生物における基本的な生物学的現象である.

研究 の 目的:

  • シアノバクテリアの昼間振動器の分子メカニズムを解明する.
  • KaiA,KaiB,およびKaiCのタンパク質がどのように相互作用して昼夜リズムを生成するかを理解する.
  • KaiABCオシレータの片方向のティッキングの構造的基礎を調査するために.

主な方法:

  • カイA,カイB,カイCタンパク質を用いて,サーカディアンオシレータの試験管内再構成.
  • コアオシレータタンパク質の高解像度構造分析.
  • タンパク質の相互作用とリン酸化現象を研究するための生体物理および生体化学分析.

主要な成果:

  • KaiABCタンパク質複合体は,生化学的な振動器を形成し,in vitroで昼夜時刻を計測することができる.
  • 構造的なデータは,一方向の振動を駆動するラッチングメカニズムを示唆しています.

さらに関連する動画

Monitoring Cell-autonomous Circadian Clock Rhythms of Gene Expression Using Luciferase Bioluminescence Reporters
10:38

Monitoring Cell-autonomous Circadian Clock Rhythms of Gene Expression Using Luciferase Bioluminescence Reporters

Published on: September 27, 2012

Manipulation of Rhythmic Food Intake in Mice Using a Custom-Made Feeding System
07:34

Manipulation of Rhythmic Food Intake in Mice Using a Custom-Made Feeding System

Published on: December 16, 2022

関連する実験動画

Last Updated: Jun 28, 2026

Parallel Measurement of Circadian Clock Gene Expression and Hormone Secretion in Human Primary Cell Cultures
06:53

Parallel Measurement of Circadian Clock Gene Expression and Hormone Secretion in Human Primary Cell Cultures

Published on: November 11, 2016

Monitoring Cell-autonomous Circadian Clock Rhythms of Gene Expression Using Luciferase Bioluminescence Reporters
10:38

Monitoring Cell-autonomous Circadian Clock Rhythms of Gene Expression Using Luciferase Bioluminescence Reporters

Published on: September 27, 2012

Manipulation of Rhythmic Food Intake in Mice Using a Custom-Made Feeding System
07:34

Manipulation of Rhythmic Food Intake in Mice Using a Custom-Made Feeding System

Published on: December 16, 2022

  • リン酸化現象と形状の変化は,振動器の相を決定する鍵です.

    • 結論:

    • KaiABC振動器は,翻訳後の生物学的タイムキーピングのための基本的なモデルを提供します.
    • この振動器は, in vivo のトランスクリプションとトランスレーションのフィードバックループと統合される可能性が高い.
    • 構造的,生体物理的,生化学的アプローチの組み合わせは,昼夜メカニズムを理解するために不可欠です.