HITS-CLIPは,脳の代替RNA処理に関する全ゲノムの洞察をもたらしています
Donny D Licatalosi1, Aldo Mele, John J Fak
1Laboratory of Molecular Neuro-Oncology and Howard Hughes Medical Institute, The Rockefeller University, 1230 York Avenue, New York, New York 10021, USA.
Nature
|November 4, 2008
まとめ
クロスリンキング免疫プレシピテーション (HITS-CLIP) によって分離されたRNAの高通量配列化は,タンパク質-RNAの相互作用を in vivo でマップします. この方法は,Novaスプライシングファクターが脳の代替スプライシングとポリアデニレーションを含む遺伝子発現をどのように制御するかを明らかにします.
科学分野:
- 分子生物学は分子生物学である.
- 遺伝学 遺伝学とは
- 神経科学は神経科学である.
背景:
- タンパク質とRNAの相互作用は,遺伝子発現において極めて重要です.
- 生化学的方法を使用して生体組織におけるこれらの相互作用を研究することは困難です.
研究 の 目的:
- タンパク質-RNA結合部位を in vivoでマッピングするための全ゲノム方法を開発する.
- ニューロン特異のスプライシングファクターNova.のインビボRNA結合環境を調査する.
主な方法:
- クロスリンキング免疫プレシピテーション (HITS-CLIP) によって分離されたRNAの高通量シーケンシング.
- ネズミの脳におけるNova-RNA相互作用の分析.
主要な成果:
- HITS-CLIPは,Nova in vivoのための複製可能な全ゲノムRNA結合マップを生成しました.
- これらのマップは,代替スプライシングの結果を決定するNovaの役割を確認し,その行動を de novoで予測しました.
- 3'未翻訳領域で多数のNova-RNA相互作用が特定され,脳内の代替ポリアデニレーションのNovaの調節を明らかにした.
結論:
- HITS-CLIPは,in vivoで機能性タンパク質-RNA相互作用を特定するための堅牢で偏見のない方法です.
- この技術は,Nova.のようなスプライシング因子による遺伝子発現の調節に関する新しい洞察を提供します.
関連する概念動画
Alternative RNA Splicing
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Alternative RNA Splicing
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
RNA-seq
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...
Ribosome Profiling
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...
Pre-mRNA Processing: RNA Splicing
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA Splicing
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...


