ドロソフィラ菌の熱ショック因子の協同結合は,保存された5bpのユニットの配列に結合する
1Section of Biochemistry, Molecular and Cell Biology, Cornell University, Ithaca, New York 14853.
Cell
|February 8, 1991
まとめ
ドロソフィラの熱ショック因子 (HSF) は,DNAを協力的に結合するマルチメーターを形成します. この協力性は,ヒートショックの遺伝子調節に不可欠な複数のサイトへのHSF結合を強化します.
科学分野:
- 分子生物学は分子生物学である.
- 遺伝学 遺伝学とは
- バイオケミストリー バイオケミストリー
背景:
- ドロソフィラ熱ショック因子 (HSF) は,溶液中のマルチマーとして存在し,その調節要素 (熱ショック要素 - HSE) と結合すると存在します.
- 以前の研究では,HSFサブユニットがホモトリマーに結合し,各サブユニットはHSE内の保存された5bpのDNA配列に接触することを示しました.
研究 の 目的:
- HSFの2つの異なるレベルでの協力結合を調査する:マルチマー内のサブユニット間,および複数のHSFマルチマー間.
- 37°Cでの結合におけるHSFの協力性の役割とそのインビボ結合要件と差異的な熱ショック遺伝子発現への影響を理解する.
主な方法:
- ドロソフィラの熱ショック因子 (HSF) マルチメリゼーションとDNA結合の実験分析.
- 隣接するHSF結合サイトにおける協力的な結合親和性の評価.
主要な成果:
- HSFの結合は,HSFの複数体内および複数体間の2つのレベルで高い協力性を示しています.
- HSFを2つの隣接するトリメア結合部位の1つに結合させると,2番目の部位へのHSF結合が2000倍以上増加する.
- この協力性は,特に37°Cで顕著です.
結論:
- HSFの結合で観察される高い協力性は,その機能にとって重要です.
- この協力性は,複数のHSEの必要性を in vivo で説明する可能性がある.
- また,熱ストレスへの反応として熱ショック遺伝子の差異的発現にも貢献します.
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