Ypt1とSec4の膜結合がBet2に依存していること
1Department of Cell Biology, Yale University School of Medicine, New Haven, Connecticut 06510.
Nature
|May 9, 1991
まとめ
BET2は,Ypt1およびSec4タンパク質の膜結合に不可欠な新たに特定された遺伝子です. このタンパク質プレニルトランスフェラーゼの改変は,酵母における膀輸送に不可欠である.
科学分野:
- 細胞生物学 細胞生物学
- 分子生物学は分子生物学である.
- バイオケミストリー バイオケミストリー
背景:
- 小型のGTP結合タンパク質は,膜結合のために,翻訳後の修正を必要とします.
- Ypt1およびSec4タンパク質は,分泌経路における膀輸送特異性を調節する.
- BET2は当初,Golgi輸送へのエンドプラズマ網膜に関与する遺伝子として特定されました.
研究 の 目的:
- Ypt1とSec4の膜結合の文脈でBET2の機能を調査する.
- BET2がYpt1とSec4.4の翻訳後の改変に作用するかどうかを判断する.
- GTP結合タンパク質の膜結合のメカニズムを理解する.
主な方法:
- 酵母におけるBET2の遺伝子解析.
- BET2とその同類体のDNA配列分析.
- Ypt1とSec4の膜結合に関する機能的研究.
主要な成果:
- BET2は,Ypt1とSec4.4の膜結合に不可欠な因子をコードする.
- BET2は,タンパク質プレニルトランスフェラーゼの成分であるDpr1 (Ram1) に同型である.
- これは,BET2がYpt1とSec4をDpr1がRasを改変するように改変することを示唆しています.
結論:
- BET2はYpt1とSec4の膜局所化に不可欠な成分である.
- BET2は,タンパク質プレニルトランスフェラーゼとして機能し,Dpr1.1と類似している可能性が高い.
- この発見は,GTP結合タンパク質の改変による膀輸送の調節に関する新しい洞察を提供します.
関連する概念動画
Lipids as Anchors
In the plasma membrane, the lipids forming the bilayer can also act as an anchor to tether proteins to the membrane. The three main types of lipid anchors found in eukaryotes are – prenyl groups, fatty acyl groups, and glycosylphosphatidylinositol or GPI groups. Prenyl and fatty acyl groups act as anchors on the cytosolic surface of the membrane, whereas GPI anchors proteins on the extracellular side.
The carboxy-terminal of most of the prenylated proteins, such as Ras proteins, contains the...
The carboxy-terminal of most of the prenylated proteins, such as Ras proteins, contains the...
Septins
Septins are protein filaments forming the cytoskeleton along with the microtubules, microfilaments, intermediate filaments, and other accessory proteins. In 1971 while studying the cell division cycle in mutant Saccharomyces cerevisiae Harwell et al. first identified the septin-related genes playing a crucial role in yeast cytokinesis. Fluorescence microscopy revealed that these proteins localize at the budding neck as rings. These ring-like proteins were then named Septins by John Pringle, and...
Coat Assembly and GTPases
Vesicles incorporate different coat protein subunits in different cell locations, which changes the properties of the coat, such as the shape and geometry of the transport vesicles. Thus, vesicle coat proteins also play a significant role in cargo selection.
Coat assembly depends on the local availability of phosphatidylinositol phosphates or PIPs and GTP-binding proteins. Adaptor proteins, which link the coat proteins to the membrane, bind to these PIPs and play a crucial role in controlling...
Coat assembly depends on the local availability of phosphatidylinositol phosphates or PIPs and GTP-binding proteins. Adaptor proteins, which link the coat proteins to the membrane, bind to these PIPs and play a crucial role in controlling...
Cotranslational Protein Translocation
Translocation of proteins across membranes is an ancient process that occurs even in bacteria and archaebacteria. In fact, the components of the translocation machinery are still conserved between prokaryotes and eukaryotes.
Sec61 channel partners for cotranslational translocation
During cotranslational translocation, the Sec61 channel partners with the signal recognition particle (SRP), the signal recognition particle receptor (SR), and the ribosomes to transport the nascent polypeptide chain...
Sec61 channel partners for cotranslational translocation
During cotranslational translocation, the Sec61 channel partners with the signal recognition particle (SRP), the signal recognition particle receptor (SR), and the ribosomes to transport the nascent polypeptide chain...
Tail-anchoring of Proteins in the ER Membrane
Tail-anchored, or TA, proteins are estimated to make up to 3-5% of membrane proteins found in the eukaryotic cell. Such proteins have a single transmembrane domain located approximately 30 amino acid residues upstream from the C-terminal end. As a result, the signal recognition particle (SRP) cannot guide a TA protein to the ER membrane for cotranslational insertion. Hence, they are integrated into the ER membrane post-translationally using their C-terminal end as the anchor. TA proteins...
Bacterial Translocation and Protein Secretion
Bacterial protein secretion involves translocation systems to ensure proteins reach their designated locations, including the plasma membrane, periplasm, outer membrane, or the external environment. These translocation systems are vital for bacterial physiology, supporting processes like membrane assembly, enzymatic activity in the periplasm, and interactions with the external environment. The division of labor between Sec and Tat pathways ensures efficiency in handling proteins with diverse...


