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関連する概念動画

siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...
Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
Small interfering RNAs (siRNA)02:30

Small interfering RNAs (siRNA)

Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...
Cell Specific Gene Expression01:58

Cell Specific Gene Expression

Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...

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関連する実験動画

Updated: Jun 27, 2026

Cell Based Assays of SINEUP Non-coding RNAs That Can Specifically Enhance mRNA Translation
10:21

Cell Based Assays of SINEUP Non-coding RNAs That Can Specifically Enhance mRNA Translation

Published on: February 1, 2019

人間の細胞の反意味トランスクリプトームです.

Yiping He1, Bert Vogelstein, Victor E Velculescu

  • 1Ludwig Center for Cancer Genetics and Therapeutics and Howard Hughes Medical Institute, Johns Hopkins Kimmel Cancer Center, Baltimore, MD 21231, USA.

Science (New York, N.Y.)
|December 6, 2008
PubMed
まとめ
この要約は機械生成です。

研究者は,特定のDNA鎖からRNAトランスクリプトを識別し,定量化するための新しい技術を開発しました. この方法により,ヒトの細胞に浸透した反感覚トランスクリプトが発見され,遺伝子調節における重要な役割が示唆された.

さらに関連する動画

Transcriptome Analysis of Single Cells
07:27

Transcriptome Analysis of Single Cells

Published on: April 25, 2011

関連する実験動画

Last Updated: Jun 27, 2026

Cell Based Assays of SINEUP Non-coding RNAs That Can Specifically Enhance mRNA Translation
10:21

Cell Based Assays of SINEUP Non-coding RNAs That Can Specifically Enhance mRNA Translation

Published on: February 1, 2019

Transcriptome Analysis of Single Cells
07:27

Transcriptome Analysis of Single Cells

Published on: April 25, 2011

科学分野:

  • 分子生物学は分子生物学である.
  • ゲノミクスゲノミクスとは
  • 遺伝子発現分析 遺伝子発現分析

背景:

  • ゲノム全体の転写分析が確立されていますが,DNA鎖からRNA転写原点 (意味と反意味) を区別することは困難です.
  • 感覚と反感覚のトランスクリプトの関係を理解することは,遺伝子規制メカニズムを明らかにするために重要です.

研究 の 目的:

  • RNAトランスクリプトの起源のDNA鎖を特定するための新しい技術を開発し,検証する.
  • 人間の細胞で発現する遺伝子からの感覚と反感覚のトランスクリプトをグローバルに定量化するために.

主な方法:

  • 個々のRNAトランスクリプトのDNA鎖の起源を決定するための新しい技術が開発されました.
  • この方法は,5種類のヒト細胞の感覚と反感覚のトランスクリプトをグローバルに定量化するために適用されました.

主要な成果:

  • この技術は,RNAトランスクリプトの起源であるDNA鎖を成功裏に特定しました.
  • アンチセンストランスクリプトの証拠は,調査したすべての細胞タイプで2900から6400のヒト遺伝子で見つかった.
  • アンチセンストランスクリプトの分布は感覚トランスクリプトとは異なり,ゲノム全体で非ランダムで,細胞タイプによって異なる.

結論:

  • アンチセンセスのトランスクリプトは,ヒト細胞の至る所にある特徴です.
  • アンチセンセスのトランスクリプトは,ヒトの遺伝子調節の根本的な構成要素である可能性が高い.