関連する実験動画
Updated: Jun 27, 2026

09:09
An Efficient and High Yield Method for Isolation of Mouse Dendritic Cell Subsets
Published on: April 18, 2016
CD74によるデンドリット細胞移動の調節,MHCクラスIIに関連する不変連鎖であるCD74によるデンドリット細胞移動の調節
Gabrielle Faure-André1, Pablo Vargas, Maria-Isabel Yuseff
1INSERM U653, Institut Curie, 12 rue Lhomond, 75005, Paris, France.
まとめ
インヴァリアントチェーン (IiまたはCD74) は,デンドリット細胞 (DC) の動きを調節する. Ii欠乏はDCの運動性を高め,抗原処理と組織内の免疫細胞の移動との関連を示唆する.
科学分野:
- 免疫学 免疫学とは
- 細胞生物学 細胞生物学
- バイオフィジックス 生物物理学
背景:
- dendritic 細胞 (DCs) は,周辺組織から抗原を採取することによって,適応性免疫反応を開始するために不可欠です.
- 抗原処理とDC運動性は,伝統的に独立したプロセスと考えられてきました.
- インヴァリアントチェーン (IIまたはCD74) は,MHCクラスII分子によるプレゼンテーションのための抗原処理を調節することが知られている.
研究 の 目的:
- dendritic cell (DC) の運動を調節するインヴァリアントチェーン (IiまたはCD74) の役割を調査する.
- 抗原処理とDC運動性が関連しているかどうかを判断する.
- IiがDC移行に影響を与える分子メカニズムを理解する.
主な方法:
- マイクロファブリックチャネルを使って,外周組織の狭いマイクロ環境をシミュレートしました.
- ワイルド型DCの運動性を,これらのチャネルにおけるII欠乏型DCと比較した.
- DC運動のII媒介調節におけるミオシンIIの関与を調査した.
主要な成果:
- 野生型のDCは,閉じ込められた環境で,交互に高低機動性の期間を示した.
- Ii欠陥のDCは,ワイルド型細胞と比較して,より速く,より均一な動きを示した.
- IiのDC運動に対する規制効果は,アクチンベースのモータータンパク質,ミオシンIIに依存していた.
結論:
- インヴァリアントチェーン (IiまたはCD74) は,体内では dendritic 細胞の運動性を否定的に調節する.
- アンチゲン処理と細胞運動は,DCsのカップリングされたプロセスで,IIとミオシンIIによって媒介されます.
- この結合は,組織空間内のDCによる抗原検出と処理の効率を高める可能性があります.
さらに関連する動画
関連する概念動画
T Cell Activation and Clonal Selection
T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
Naive T cells that have not yet encountered an antigen express two primary CD...
Antigen Processing Pathways
MHC molecules are key players in the immune response, enabling T cells to recognize and respond to specific antigens. They are present on the surface of all nucleated cells in the body and are instrumental in presenting antigens to T cells and activating them. T cells recognize the MHC-antigen complex and initiate an immune response. MHC class I and MHC class II are two main types of MHC molecules, each associated with a distinct antigen processing pathway.
MHC Class I: Presenting Endogenous...
MHC Class I: Presenting Endogenous...
Antigens Involved in Adaptive Immunity
An antigen is any substance the immune system identifies as foreign and potentially harmful to the body, prompting an immune response. Antigens have two functional properties: immunogenicity and reactivity. Immunogenicity is the ability of an antigen to stimulate a specific immune response. At the same time, reactivity describes the antigen's ability to react with the cells and antibodies produced in response to it.
Complete Antigens
Complete antigens possess both immunogenicity and reactivity.
Complete Antigens
Complete antigens possess both immunogenicity and reactivity.
Immunoglobulin-like Cell Adhesion Molecules
Immunoglobulin-like cell adhesion molecules or Ig-CAMs are a versatile group of cell surface glycoproteins belonging to the immunoglobulin protein superfamily. Ig-CAMs possess the characteristic immunoglobulin protein domains and other domains such as the fibronectin type III domain. The Ig domains are glycosylated to varying degrees in different Ig-CAMs.
Ig-CAMs exhibit either homophilic binding (to other Ig-CAMs) or heterophilic binding (to other ligands such as integrins). While most Ig-CAMs...
Ig-CAMs exhibit either homophilic binding (to other Ig-CAMs) or heterophilic binding (to other ligands such as integrins). While most Ig-CAMs...
Receptor Downregulation in MVBs
Multivesicular bodies (MVBs) are mature endosomes that sort ubiquitinated proteins and then fuse with lysosomes to degrade the sorted proteins. Epidermal growth factor (EGF) and its receptor (EGFR) form a complex that can be internalized through endocytosis, sorted into an MVB, and later degraded.
The EGFR can initiate signaling pathways that lead to cell proliferation, migration, and differentiation. Overexpression of EGFR stimulates cells to proliferate. Excessive EGFR activation may...
The EGFR can initiate signaling pathways that lead to cell proliferation, migration, and differentiation. Overexpression of EGFR stimulates cells to proliferate. Excessive EGFR activation may...
T Cell Types and Functions
When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...

