トランスクリプションの活性化を媒介するTATA結合タンパク質と関連したコアクティベーターを分離する
B D Dynlacht1, T Hoey, R Tjian
1Howard Hughes Medical Institute, Department of Molecular and Cell Biology, University of California, Berkeley 94720.
Cell
|August 9, 1991
まとめ
ドロソフィラの転写因子NTF-1の活性化には,TBPだけでなく,TFIID関連因子 (TAFs) が必要です. TAFは,基礎活性とは異なる,NTF-1-誘導転写のための重要な共同活性化剤である.
科学分野:
- 分子生物学は分子生物学である.
- 発達生物学 発達生物学について
- 遺伝学 遺伝学とは
背景:
- トランスクリプションの調節は,発達にとって極めて重要です.
- プロモーター選択因子は,転写機構と相互作用する.
- NTF-1は,ドロソフィラ菌における重要な発達的に調節される転写因子である.
研究 の 目的:
- NTF-1による転写活性化の要件を解明する.
- NTF-1機能に関与するトランスクリプション装置の特定のコンポーネントを特定する.
主な方法:
- 分割されたドロソフィラ基底因子を用いて再構成された転写アッセイ.
- TFIID複合体の組成を分析するために,グリセロールの梯度沈殿.
- TBP関連因子 (TAF) を隔離し,特徴付けるための免疫プレシピテーション.
主要な成果:
- NTF-1の活性化には,浄化されたTATA結合タンパク質 (TBP) と異なるTFIID分数の内の要素が必要です.
- TFIIDは,TBPと少なくとも6つのTBP関連因子 (TAF) の複合体です.
- TAFsが欠けているTBPは基礎転写をサポートしますが,NTF-1の活性化をサポートしません. TAFsを追加すると活性化が回復します.
結論:
- TBP関連因子 (TAFs) は,NTF-1-媒介の転写活性化のための重要な共同活性化因子である.
- 特定のTAFポリペプチドは,コアクティベーター機能を付与し,基礎転写を超えてその役割を強調しています.
- この研究は,ドロソフィラ菌における発達の転写因子活性化の分子メカニズムを明らかにしています.
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