血液形成性幹細胞のニッチ形成には,内分泌体骨化が必要である
Charles K F Chan1, Ching-Cheng Chen, Cynthia A Luppen
1Department of Pathology, Developmental Biology and Institute for Stem Cell Biology and Regenerative Medicine, Stanford University, California, USA. chazchan@stanford.edu
Nature
|December 17, 2008
まとめ
成人型造血幹細胞 (HSC) のニッチ形成には,内分泌骨格化が必要である. 特定の胎児の祖先細胞は,血管の徴募と骨髄腔の生成を駆動し,HSCの維持に不可欠です.
科学分野:
- 発達生物学 発達生物学について
- 幹細胞生物学 幹細胞生物学
- ヘマトポエーシス (血液形成) とは
背景:
- 幹細胞の維持は,ニッチと呼ばれる特定のマイクロ環境に依存しています.
- 血液形成幹細胞 (HSC) のニッチ形成の過程はよく理解されていません.
研究 の 目的:
- 成人HSCニッチ形成のインビボアッセイを開発する.
- HSCのニッチ開発に不可欠な原始細胞集団を特定する.
主な方法:
- 成人マウスの腎臓カプセルの下に胎児の骨の原始細胞の移植を含むインビボ測定を用いた.
- 表面マーカー (CD45,Tie2,alphaV,CD105,Thy1.1) を基に並べられた原始細胞.
- ニッチ形成における内分泌骨格化因子 (オステリックス,VEGF) の役割を調査した.
主要な成果:
- 特定されたCD105 ((+) Thy1 ((-) 祖先細胞は,骨髄腔を持つ子宮外骨を形成し,長期的なHSCをサポートします.
- CD105 (((+) Thy1 (((+) プロジェニタが,髄腔がない骨を形成することを実証しました.
- 腸内軟骨の骨化因子を抑制することで,ニッチ生成を抑制することが示された.
- 骨格化領域以外の内分泌体からの祖先は,骨髄腔を形成しないことが確認されました.
結論:
- 軟骨介質を介したプロセスであるエンドコンドラの骨化が,成人のHSCのニッチ形成に不可欠である.
- 特定の原始細胞集団 (CD105 ((+)) Thy1 ((-)) は,内分泌骨格化によるHSCのニッチ開発の主要な原動力である.
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