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関連する概念動画

Covalently Linked Protein Regulators02:04

Covalently Linked Protein Regulators

Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein.
Restarting Stalled Replication Forks02:37

Restarting Stalled Replication Forks

DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart, a...
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
Riboswitches01:56

Riboswitches

Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
Covalently Linked Protein Regulators02:04

Covalently Linked Protein Regulators

Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein.
Transcriptional Regulation: Riboswitches01:23

Transcriptional Regulation: Riboswitches

Riboswitches are RNA elements that regulate gene expression by altering their secondary structures in response to specific effector molecules. These elements, located in the leader regions of certain mRNAs, act as transcriptional regulators by toggling between alternative conformations to control downstream gene expression. Riboswitch-mediated regulation is a precise mechanism for modulating biosynthetic pathways, as exemplified by the riboflavin biosynthesis pathway in Bacillus...

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In Vivo Detection and Analysis of Rb Protein SUMOylation in Human Cells
09:40

In Vivo Detection and Analysis of Rb Protein SUMOylation in Human Cells

Published on: November 2, 2017

SUMOylationは,Rad18媒介のテンプレートスイッチを調節する.

Dana Branzei1, Fabio Vanoli, Marco Foiani

  • 1IFOM, the FIRC Institute for Molecular Oncology Foundation, IFOM-IEO Campus, Via Adamello 16, 20139 Milan, Italy. dana.branzei@ifom-ieo-campus.it

Nature
|December 19, 2008
PubMed
まとめ
この要約は機械生成です。

この研究では,Rad18タンパク質は,酵母における複製フォークにおけるDNA損傷バイパスに不可欠であり,PCNAユビキティレーションとSUMOylation経路によるテンプレートスイッチングと再結合を調整していることが明らかになりました.

さらに関連する動画

In Vitro SUMOylation Assay to Study SUMO E3 Ligase Activity
09:45

In Vitro SUMOylation Assay to Study SUMO E3 Ligase Activity

Published on: January 29, 2018

Real-time Observation of the DNA Strand Exchange Reaction Mediated by Rad51
06:24

Real-time Observation of the DNA Strand Exchange Reaction Mediated by Rad51

Published on: February 13, 2019

関連する実験動画

Last Updated: Jun 25, 2026

In Vivo Detection and Analysis of Rb Protein SUMOylation in Human Cells
09:40

In Vivo Detection and Analysis of Rb Protein SUMOylation in Human Cells

Published on: November 2, 2017

In Vitro SUMOylation Assay to Study SUMO E3 Ligase Activity
09:45

In Vitro SUMOylation Assay to Study SUMO E3 Ligase Activity

Published on: January 29, 2018

Real-time Observation of the DNA Strand Exchange Reaction Mediated by Rad51
06:24

Real-time Observation of the DNA Strand Exchange Reaction Mediated by Rad51

Published on: February 13, 2019

科学分野:

  • 分子生物学は分子生物学である.
  • 遺伝学 遺伝学とは
  • バイオケミストリー バイオケミストリー

背景:

  • テンプレートスイッチングは,複製中のDNA損傷バイパスとギャップ埋めるための重要なメカニズムです.
  • ギャップ埋める修復には,Rad18とRad5.5によって媒介される増殖細胞核抗原 (PCNA) の同類の再結合とポリユビキチル化が含まれます.
  • これらの修復経路と,レプリケーションフォークでテンプレート切り替えの物理的な証拠の間の調整は不明である.

研究 の 目的:

  • DNAダメージバイパス,同類再結合,およびPCNAの汎用化の間の調整を調査する.
  • レプリケーションフォークでRad18-Rad5-依存のテンプレートスイッチングの物理的な証拠を提供するために.
  • 複製結合再結合におけるPCNAユビキティレーションとSUMOylationの役割を解明する.

主な方法:

  • Saccharomyces cerevisiae.における遺伝的アプローチについて
  • X形姉妹染色体結合 (SCJs) の物理的検出.
  • PCNAポリユビキチル化とSUMOylation経路の分析. PCNAポリユビキチル化とSUMOylation経路の分析. PCNAポリユビキチル化とSUMOylation経路の分析. PCNAポリユビキチル化とSUMOylation経路の分析. PCNAポリユビキチル化とSUMOylation経路の分析.

主要な成果:

  • Rad18は,損傷した複製フォークでSCJを形成するために不可欠であり,PCNAのMms2とUbc13経由のポリユビキチル化を伴う.
  • SCJ経由のダメージバイパスには,Ubc9,SUMOylated PCNAが必要であり,Rad51-依存リコンビネーションと連携しています.
  • この研究は,Rad18-Rad5-Mms2依存型SCJがテンプレートスイッチイベントを表すことを示唆している.

結論:

  • PCNAユビキティレーションとSUMOylation経路は,一時的な,ダメージ誘発,複製結合再結合イベントを促進するために重要です.
  • 複製フォークの姉妹染色体結合は,これらの再結合イベントで役割を果たします.
  • この研究は,テンプレート・スイッチングと再結合を含むDNA損傷耐性の協調メカニズムを明らかにしています.