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関連する概念動画

Regulation of Expression Occurs at Multiple Steps02:24

Regulation of Expression Occurs at Multiple Steps

Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
Leaky Scanning02:28

Leaky Scanning

During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA.  Marilyn Kozak discovered that the sequence RCCAUGG (where R stands for...
Regulation of the Unfolded Protein Response01:31

Regulation of the Unfolded Protein Response

Inositol-requiring kinase one or IRE1 is the most conserved eukaryotic unfolded protein response (UPR) receptor. It is a type I transmembrane protein kinase receptor with a distinctive site-specific RNase activity. As the binding mechanics of the misfolded proteins with the N-terminal domain of IRE-1 are unclear, three binding models — direct, indirect, and allosteric -- are proposed for receptor activation. Nevertheless, it is known that once a misfolded protein associates with IRE1, it...
Regulation of Angiogenesis and Blood Supply01:24

Regulation of Angiogenesis and Blood Supply

Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits.  Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl hydroxylase and factor...
Regulation of Expression Occurs at Multiple Steps02:24

Regulation of Expression Occurs at Multiple Steps

Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
TGF - β Signaling Pathway01:16

TGF - β Signaling Pathway

The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors are of three kinds RI, RII, and RIII. The RI...

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Updated: Jun 21, 2026

A Simple Bioassay for the Evaluation of Vascular Endothelial Growth Factors
09:04

A Simple Bioassay for the Evaluation of Vascular Endothelial Growth Factors

Published on: March 15, 2016

ストレス反応性RNAスイッチは,VEGFA発現を調節する.

Partho Sarothi Ray1, Jie Jia, Peng Yao

  • 1Department of Cell Biology, The Lerner Research Institute, Cleveland Clinic, 9500 Euclid Avenue, Cleveland, Ohio 44195, USA.

Nature
|December 23, 2008
PubMed
まとめ
この要約は機械生成です。

研究者らは,ヒトの血管内皮成長因子-A (VEGFA) mRNAにおける新しいRNAスイッチを発見した. このスイッチは,インターフェロン-ガンマと低酸素信号を統合して,骨髄細胞におけるVEGFA翻訳を制御します.

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Overexpressing Long Noncoding RNAs Using Gene-activating CRISPR
13:04

Overexpressing Long Noncoding RNAs Using Gene-activating CRISPR

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関連する実験動画

Last Updated: Jun 21, 2026

A Simple Bioassay for the Evaluation of Vascular Endothelial Growth Factors
09:04

A Simple Bioassay for the Evaluation of Vascular Endothelial Growth Factors

Published on: March 15, 2016

Molecular Analysis of Endothelial-mesenchymal Transition Induced by Transforming Growth Factor-&#946; Signaling
07:49

Molecular Analysis of Endothelial-mesenchymal Transition Induced by Transforming Growth Factor-β Signaling

Published on: August 3, 2018

Overexpressing Long Noncoding RNAs Using Gene-activating CRISPR
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Overexpressing Long Noncoding RNAs Using Gene-activating CRISPR

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科学分野:

  • 分子生物学は分子生物学である.
  • 遺伝子規制 遺伝子規制
  • RNA 生物学 RNA 生物学

背景:

  • 非コーディングRNA領域は,リガンド結合を通じて遺伝子発現を調節する.
  • リボスイッチやその他のRNAスイッチは,代謝産物やタンパク質に結合した代謝産物に対して反応する.
  • これらのシステムは,細菌,真菌,植物に見られます.

研究 の 目的:

  • 人間の血管内皮成長因子-A (VEGFA) の新しいRNAスイッチを特定し,特徴づけること. mRNA.
  • このスイッチがどのように環境信号を統合して遺伝子発現を調節するかを調査する.
  • ミエロイド細胞におけるVEGFA翻訳調節のメカニズムを理解する.

主な方法:

  • VEGFA mRNA 3' 未翻訳領域 (UTR) の分析.
  • 刺激に対する反応におけるVEGFA 3' UTRへのタンパク質結合の調査.
  • RNAスイッチにおける構造変化の特徴.

主要な成果:

  • 人間のVEGFA mRNA 3' UTR.でメタボライト独立のRNAスイッチが特定されました.
  • このスイッチは,インターフェロン (IFN) -ガンマと低酸素からの信号を統合します.
  • スイッチの構成の変化は,IFN-ガンマ活性化トランスレーション複合体および異質核リボ核タンパク質L (HNRNPL) の阻害剤の相互排他的結合によって制御されます.

結論:

  • VEGFA mRNA 3' UTRは新しいRNAスイッチとして作用し,骨髄細胞におけるVEGFA翻訳を調節する.
  • このスイッチはタンパク質に依存し,複数の環境信号を統合します.
  • それは,多細胞動物における遺伝子調節のために進化したRNAスイッチの新しいクラスを表す可能性があります.