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関連する概念動画

Protein Denaturation01:28

Protein Denaturation

The function of proteins depends on their native three-dimensional structure, which is dictated by the amino acid sequence of the specific protein. Folding of the polypeptide chain takes place under specific conditions that energetically favor the folded conformation. In contrast, protein denaturation occurs spontaneously under unfavorable conditions that disrupt the integrity of the folded conformation. Thus, the chemical and physical environment of a protein, such as significant changes in pH...
Phase Transitions: Melting and Freezing02:39

Phase Transitions: Melting and Freezing

Heating a crystalline solid increases the average energy of its atoms, molecules, or ions, and the solid gets hotter. At some point, the added energy becomes large enough to partially overcome the forces holding the molecules or ions of the solid in their fixed positions, and the solid begins the process of transitioning to the liquid state or melting. At this point, the temperature of the solid stops rising, despite the continual input of heat, and it remains constant until all of the solid is...
Entropy and Solvation02:05

Entropy and Solvation

The process of surrounding a solute with solvent is called solvation. It involves evenly distributing the solute within the solvent. The rule of thumb for determining a solvent for a given compound is that like dissolves like. A good solvent has molecular characteristics similar to those of the compound to be dissolved. For example, polar solutions dissolve polar solutes, and apolar solvents dissolve apolar solutes. A polar solvent is a solvent that has a high dielectric constant (ϵ ≥ 15); an...
DNA Isolation01:34

DNA Isolation

DNA from cells is required for many biotechnology and research applications, such as molecular cloning. To remove and purify DNA from cells, researchers use various methods of DNA extraction. While the specifics of different protocols may vary, some general concepts underlie the process of DNA extraction.
¹H NMR of Conformationally Flexible Molecules: Variable-Temperature NMR01:15

¹H NMR of Conformationally Flexible Molecules: Variable-Temperature NMR

The axial and equatorial protons in cyclohexane can be distinguished by performing a variable-temperature NMR experiment. In this process, except for one proton, the remaining eleven protons are replaced by deuterium. The deuterium substitution avoids the possible peak splitting caused by the spin-spin coupling between the adjacent protons. The remaining proton flips between the axial and equatorial positions.
Recrystallization: Solid–Solution Equilibria01:10

Recrystallization: Solid–Solution Equilibria

Recrystallization is a purification technique used to separate impurities from solid compounds. In this technique, no chemical reactions occur. Instead, it exploits physical properties only, specifically, the solubility differences between the desired compound and impurities, either at a single temperature or at different temperatures, and under other selected conditions. The solid-solution equilibrium (solubility equilibrium) of each component in the solution represents a binary phase...

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関連する実験動画

Updated: Jun 26, 2026

Determination of Protein-ligand Interactions Using Differential Scanning Fluorimetry
13:26

Determination of Protein-ligand Interactions Using Differential Scanning Fluorimetry

Published on: September 13, 2014

溶媒から見たように,タンパク質の冷凍デナチュレーション.

Monika Davidovic1, Carlos Mattea, Johan Qvist

  • 1Department of Biophysical Chemistry, Center for Molecular Protein Science, Lund University, SE-22100 Lund, Sweden.

Journal of the American Chemical Society
|January 1, 2009
PubMed
まとめ

球状タンパク質の冷凍デナチュレーションは,水-17) Oスピンリラクゼーションを用いて調査されました. アポミオグロビンは冷変性化され,タンパク質と水の相互作用や冷変性化のメカニズムに関する一般的な仮定に異議を唱えました.

科学分野:

  • バイオフィジックス 生物物理学
  • タンパク質のダイナミクス
  • 熱力学は熱力学である.

背景:

  • 球状タンパク質は,ほとんどの秩序あるシステムとは異なり,最大限の安定性の温度を持っています.
  • 寒冷デナチュレーション,または冷却による構造の破壊は,通常,水害性力に関連しています.
  • 寒冷デナチュレーション中の水分化の変化の実験的調査は欠けている.

研究 の 目的:

  • 低温でタンパク質の水分化ダイナミクスを実験的に監視する.
  • さまざまな球状タンパク質の寒冷変性現象を調査する.
  • 寒冷デナチュレーションにおけるタンパク質と水の相互作用の役割を理解する.

主な方法:

  • 水-(17) Oのスピンリラクゼーションは,水分化ダイナミクスを研究するために使用されました.
  • タンパク質 (BPTI,ウビキチン,アポミオグロビン,ベータ・ラクトグローブリン) は水溶液で研究されました.
  • 溶液は,ピコリットルのエムルションドロップに含まれており, -35°Cまで氷の形成を防ぎました.

主要な成果:

  • 不安定したアポミオグロビンのみ,冷たい変性化を示した.
  • ユビキチンは -32°Cまで安定し,ベータ・ラクトグローブリンは -5°C以下では不安定だったが,運動的に閉じ込められた.

さらに関連する動画

How to Stabilize Protein: Stability Screens for Thermal Shift Assays and Nano Differential Scanning Fluorimetry in the Virus-X Project
07:22

How to Stabilize Protein: Stability Screens for Thermal Shift Assays and Nano Differential Scanning Fluorimetry in the Virus-X Project

Published on: February 11, 2019

Differential Scanning Calorimetry — A Method for Assessing the Thermal Stability and Conformation of Protein Antigen
08:13

Differential Scanning Calorimetry — A Method for Assessing the Thermal Stability and Conformation of Protein Antigen

Published on: March 4, 2017

関連する実験動画

Last Updated: Jun 26, 2026

Determination of Protein-ligand Interactions Using Differential Scanning Fluorimetry
13:26

Determination of Protein-ligand Interactions Using Differential Scanning Fluorimetry

Published on: September 13, 2014

How to Stabilize Protein: Stability Screens for Thermal Shift Assays and Nano Differential Scanning Fluorimetry in the Virus-X Project
07:22

How to Stabilize Protein: Stability Screens for Thermal Shift Assays and Nano Differential Scanning Fluorimetry in the Virus-X Project

Published on: February 11, 2019

Differential Scanning Calorimetry — A Method for Assessing the Thermal Stability and Conformation of Protein Antigen
08:13

Differential Scanning Calorimetry — A Method for Assessing the Thermal Stability and Conformation of Protein Antigen

Published on: March 4, 2017

  • 寒冷無性化状態は,コンパクトで溶媒に浸透し,完全に展開されず,非極性分子溶解性との類似性に挑戦していました.

    • 結論:

    • 冷凍デナチュレーションはタンパク質特異であり,安定性と水分による影響を受けます.
    • 観察された冷却変性状態は,完全な展開ではなく,溶媒の浸透を示唆しています.
    • いくつかのシステムで報告された冷却デナチュレーションは,温度だけではなく,低水分によるものかもしれません.