双方向プロモーターは,酵母菌で広範囲にわたる転写を生成します
Zhenyu Xu1, Wu Wei, Julien Gagneur
1European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany.
Nature
|January 27, 2009
まとめ
研究者は,酵母における広範囲の転写を分析し,安定した注釈のない転写 (SUTs) と暗号的不安定な転写 (CUTs) を特定しました. これらの非コーディングRNAは,特定のゲノム領域から発生し,プロモーターを明らかにします.
科学分野:
- ゲノミクスゲノミクスとは
- 分子生物学は分子生物学である.
- RNA 生物学 RNA 生物学
背景:
- ユカリオットのゲノムには,広範囲にわたる転写があり,安定した注釈のない転写 (SUTs) や暗号的不安定な転写 (CUTs) のような数多くの新しい非コーディングRNAを生成します.
- これらのトランスクリプトは,しばしばアノテーションされたゲノム特征と重複し,その起源と潜在的な機能に関する疑問を提起します.
- SUTとCUTを含む完全なトランスクリプトームアーキテクチャの包括的な理解は欠けている.
研究 の 目的:
- Saccharomyces cerevisiaeにおけるSUTとCUTの全ゲノム分布と配置を包括的に分析する.
- これらのトランスクリプトとプロモーターや核胞体フリー領域 (NFRs) などのゲノム特性の関係を調査する.
- ゲノム組織と遺伝子調節に対する普遍的な転写の影響を明らかにする.
主な方法:
- 様々な条件下で酵母におけるSUTとCUTの全ゲノム分析.
- エクソソーム機械変異体と異なる酵母菌株のバックグラウンドからのデータを含みます.
- アノテーションされた遺伝子とNFRsに関連したトランスクリプト開始部位のマッピング.
主要な成果:
- SUTとCUTは,異なる分布パターンを示し,しばしば,他のトランスクリプトのプロモーターまたは3'エンドと関連したNFRから開始されます.
- また,すべてのコーディングトランスクリプトの約半分は,他のプロモーターと結びついたNFRから開始されます.
- これは,プロモーターの双方向性が遺伝子調節の固有の特徴であることを示唆しています.
結論:
- この研究は,普遍的な転写と重複する転写によって特徴づけられる新しいトランスクリプトームのアーキテクチャを明らかにしています.
- 研究結果は,プロモーターは本質的に双方向性があり,広範囲にわたる重複転写に寄与することを示しています.
- この配列は,転写干渉やヒストン改変などのメカニズムを通じて,隣接する遺伝子の調整を容易にする可能性がある.
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