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The MultiBac Protein Complex Production Platform at the EMBL
Published on: July 11, 2013
広範囲に広がる双方向プロモーターは,酵母における暗号的トランスクリプトの主要な源である
Helen Neil1, Christophe Malabat, Yves d'Aubenton-Carafa
1Institut Pasteur, Unité de Génétique des Interactions Macromoléculaires, CNRS, URA2171, 75015 Paris, France.
Nature
|January 27, 2009
まとめ
研究者は酵母における暗号的不安定トランスクリプト (CUT) をマッピングし,プロモーターから発生する広範囲にわたる非ランダムなトランスクリプトを明らかにした. これは,真核のプロモーターが本質的に双方向性であり,一部のCUTが遺伝子発現を潜在的に調節することを示唆しています.
科学分野:
- 分子生物学は分子生物学である.
- ゲノミクスゲノミクスとは
- ユカリオットのトランスクリプション
背景:
- 暗号的不安定トランスクリプト (CUTs) を含む隠されたトランスクリプションは,真核生物に共通しているが,理解は薄い.
- CUTはRNAポリメラーゼIIのトランスクリプトであり,Nrd1-エクソソーム-TRAMP複合体によって急速な分解を狙っています.
- 以前の研究では,CUTの分布と流行に関する高解像度,全ゲノムにわたるデータが欠けていました.
研究 の 目的:
- Saccharomyces cerevisiaeのCUTの最初の高解像度ゲノムマップを作成する.
- CUTの起源,流行,および潜在的な機能的重要性を調査する.
- 双方向性を含むエウカリオットプロモーター活動の性質を調査する.
主な方法:
- CUTsで高度に濃縮されたRNA分子の分析.
- 遺伝子発現の3'長い連続分析 (SAGE) を深層配列化に適応した.
- CUTsの詳細な,ニュクレオチド解像度ゲノムマップの生成.
主要な成果:
- CUTは,ランダムなノイズではなく,明確に定義された転写単位から発生します.
- CUTのトランスクリプションは,主にヌクレオソームフリー領域で発生し,しばしば遺伝子プロモーター領域が重なり合う.
- CUTのサブセットはmRNAの上流で起動し,遺伝子発現における潜在的な規制的役割を示唆する.
- 特定されたCUTのほとんどは,プロモーターの異なった転写から発生し,固有の双方向性を示しています.
結論:
- ユカリオットプロモーターは本質的に双方向性があり,感覚と異なったトランスクリプトの両方を生成します.
- 異なる転写はしばしば短命のCUTを生成し,これにより,プロモーターの双方向性が以前に過小評価されていたことが説明される.
- この研究では,多数の新しいCUTが特定され,その中には遺伝子調節における潜在的な役割を持つものもあります.
関連する概念動画
The Central Dogma
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In the early 1900s, scientists discovered that DNA stores all the information needed for cellular functions and that proteins perform most of these functions. However, the mechanisms of converting genetic information into functional proteins remained unknown for many years. Initially, it was believed that a single gene is...
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Genetic transfer occurs when genetic information is passed from one organism to another. It occurs via two mechanisms: vertical gene transfer and horizontal gene transfer. Vertical gene transfer occurs when genetic information is transferred from one generation to the next, which happens much more frequently than horizontal gene transfer. Both sexual and asexual reproduction are forms of vertical gene transfer, where one or more organisms pass some or all of their genome onto their progeny.
Gene Conversion
Other than maintaining genome stability via DNA repair, homologous recombination plays an important role in diversifying the genome. In fact, the recombination of sequences forms the molecular basis of genomic evolution. Random and non-random permutations of genomic sequences create a library of new amalgamated sequences. These newly formed genomes can determine the fitness and survival of cells. In bacteria, homologous and non-homologous types of recombination lead to the evolution of new...

