PAN1:トウモロコシの非対称な細胞分裂の偏化を促進する受容体のようなタンパク質
Heather N Cartwright1, John A Humphries, Laurie G Smith
1Section of Cell and Developmental Biology, University of California San Diego, 9500 Gilman Drive, San Diego, CA 92093-0116, USA.
Science (New York, N.Y.)
|January 31, 2009
まとめ
トウモロコシのパン1遺伝子は,植物における細胞分裂の指向を導く. PAN1タンパク質は受容体として作用し,植物発育に不可欠な細胞分裂を極化するための信号を伝達します.
科学分野:
- 植物生物学 植物生物学
- 細胞生物学 細胞生物学
- 発達生物学 発達生物学について
背景:
- 細胞分裂の分極化は,真核生物の発達に不可欠である.
- 植物における細胞分裂の分極化のメカニズムは,ほとんど不明のままである.
- トウモロコシの補助母細胞 (SMCs) の非対称な細胞分裂は,ガード母細胞 (GMCs) に向かっています.
研究 の 目的:
- トウモロコシにおける非対称的なSMC分裂の偏化に伴う分子メカニズムを調査する.
- SMCの分割を指向するGMC由来信号の伝達に関与する遺伝子を特定する.
主な方法:
- トウモロコシパンの遺伝子解析 ミュータント.
- 光タグを用いたタンパク質の局所化研究.
- PAN1活動に対するフォスフォタンパク質の蓄積の分析.
主要な成果:
- トウモロコシパン1遺伝子は,SMCsのプレミトーシス分極化に不可欠です.
- PAN1は,GMCの接触部位に局所化された,ルシンに富んだリピート受容体のようなタンパク質をコードします.
- PAN1は,不活性なキナーゼドメインにもかかわらず,膜関連フォスフォタンパク質の蓄積に必要であり,信号伝導における役割を果たしていることを示しています.
結論:
- PAN1は,非対称的なSMCの分割をGMCに偏向させる外部信号を伝達する上で重要な役割を果たしています.
- この研究は,トウモロコシの口腔の発達における細胞分裂の方向性を制御する方向性信号伝達経路にPAN1を関与させている.
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