血管新生細胞は,血液生成性エンドセリウムステージを通して,血液形成細胞を生成します

Christophe Lancrin¹, Patrycja Sroczynska, Catherine Stephenson

¹Cancer Research UK Stem Cell Biology Group.

Nature

|February 3, 2009

まとめ

血管新生細胞は,一時的な血液生成内皮介質を介して血液形成細胞を生成し,二つの発達理論を結びつける. 転写因子Tal1とRunx1は,胚の発達におけるこのプロセスに不可欠である.

科学分野:

発達生物学発達生物学について
ヘマトポエーシス (血液形成) とは
幹細胞生物学幹細胞生物学

背景:

造血細胞の起源については,2つの理論が存在します. それは,ヘマンジオブラスト (バイポテンシャル・プロジェンター) とヘモゲン・エンドセリウム (血液形成の可能性のある分化性エンドセリウム細胞) です.
マウスの胚性幹細胞の分化におけるブラストコロニー形成細胞 (BL-CFCs) は,血管芽細胞の概念を支持するが,生成メカニズムは不明である.

研究の目的:

血管芽細胞が血液形成細胞を生成するメカニズムを解明する.
血球形成の発達における血球芽細胞と血球内皮の役割を調査する.
血管新生細胞と血液生成内皮の理論を結びつけるために.

主な方法:

ネズミの胚性幹細胞の試験管内微分化により,先駆体集団を特定する.
マウスの胚から細胞を培養する.
転写因子の関与の分子分析 (Tal1とRunx1).

主要な成果:

血管新生細胞は,一時的な血液生成性内皮中介体を通して,血液形成細胞を生成する.
この出血性内皮の集団は,BL-CFCの発達と,胃化するマウスの胚に存在します.
Tal1は血源性内皮の形成に不可欠であり,Runx1は血源性細胞の生成に不可欠である.

結論:

この研究は,血球芽細胞と血球内皮の理論を単一の線形発達プロセスに統合しています.
血管新生細胞は,血液生成性エンドセリウム中間体を通して,血球形成細胞を生成する.
Tal1とRunx1は,この発達経路において,異なる重要な役割を果たしています.

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