Jove
Visualize
お問い合わせ
JoVE
x logofacebook logolinkedin logoyoutube logo
JoVEについて
概要リーダーシップブログJoVEヘルプセンター
著者向け
出版プロセス編集委員会範囲と方針査読よくある質問投稿
図書館員向け
推薦の声購読アクセスリソース図書館諮問委員会よくある質問
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experimentsアーカイブ
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教員リソースセンター教員サイト
利用規約
プライバシーポリシー
ポリシー

関連する概念動画

RNA Structure01:23

RNA Structure

Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
RNA Structure01:19

RNA Structure

The basic structure of RNA consists of a string of ribonucleotides attached by phosphodiester bonds. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA) involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three...
RNA Structure01:23

RNA Structure

Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
Nucleic Acid Structure01:25

Nucleic Acid Structure

The pentose sugar in DNA is deoxyribose, while in RNA the pentose sugar is ribose. The difference between the sugars is the presence of the hydroxyl group on the ribose's second carbon and a hydrogen on the deoxyribose's second carbon. The phosphate residue attaches to the hydroxyl group of the 5′ carbon of one sugar and the hydroxyl group of the 3′ carbon of the sugar of the next nucleotide, which forms  a 5′ to 3′ phosphodiester linkage.
DNA Structure
DNA has a double-helix structure. The...
Protein Folding01:25

Protein Folding

Proteins are chains of amino acids linked together by peptide bonds. Upon synthesis, a protein folds into a three-dimensional conformation, critical to its biological function. Interactions between its constituent amino acids guide protein folding, and hence the protein structure is primarily dependent on its amino acid sequence.
Protein Structure Is Critical to Its Biological Function
Proteins perform a wide range of biological functions such as catalyzing chemical reactions, providing...
Protein Folding01:22

Protein Folding

Overview

こちらも読む

関連記事

共著者、ジャーナル、引用グラフによってこの研究に関連する記事。

並び替え
Same author

Contact-Induced Phase Separation of Alloy Catalyst to Promote Carbon Nanotube Growth.

Physical review letters·2020
Same author

Human Plasma Protein Corona of Aβ Amyloid and Its Impact on Islet Amyloid Polypeptide Cross-Seeding.

Biomacromolecules·2020
Same author

Plasmacytoid dendritic cells promote acute kidney injury by producing interferon-α.

Cellular & molecular immunology·2020
Same author

Improving the clearance of protein-bound uremic toxins using cationic liposomes as an adsorbent in dialysate.

Colloids and surfaces. B, Biointerfaces·2019
Same author

Growth kinetics of single-walled carbon nanotubes with a (2<i>n</i>, <i>n</i>) chirality selection.

Science advances·2019
Same author

The possibility of using effluent ionized calcium to assess regional citrate anticoagulation in continuous renal replacement therapy.

The International journal of artificial organs·2019

関連する実験動画

Updated: Jun 25, 2026

Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells
10:34

Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells

Published on: December 9, 2022

配列にコードされた分裂剤を用いたネイティブ型のRNA三次構造と,離散分子ダイナミクスによる精細化.

Costin M Gherghe1, Christopher W Leonard, Feng Ding

  • 1Department of Chemistry, University of North Carolina, Chapel Hill, North Carolina 27599-3290, USA.

Journal of the American Chemical Society
|February 6, 2009
PubMed
まとめ

この研究では,RNA構造を調査する実験と組み合わせた新しい計算方法が導入され,複雑なRNA三次構造を正確にモデル化されています. このアプローチは,事前の仮定なしにRNA構造を効率的に精製し,柔軟で機能的に重要なRNAの研究に役立ちます.

さらに関連する動画

Analyzing and Building Nucleic Acid Structures with 3DNA
16:24

Analyzing and Building Nucleic Acid Structures with 3DNA

Published on: April 26, 2013

RNA Secondary Structure Prediction Using High-throughput SHAPE
13:42

RNA Secondary Structure Prediction Using High-throughput SHAPE

Published on: May 31, 2013

関連する実験動画

Last Updated: Jun 25, 2026

Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells
10:34

Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells

Published on: December 9, 2022

Analyzing and Building Nucleic Acid Structures with 3DNA
16:24

Analyzing and Building Nucleic Acid Structures with 3DNA

Published on: April 26, 2013

RNA Secondary Structure Prediction Using High-throughput SHAPE
13:42

RNA Secondary Structure Prediction Using High-throughput SHAPE

Published on: May 31, 2013

科学分野:

  • バイオケミストリー バイオケミストリー
  • コンピュータ生物学 コンピュータ生物学
  • 構造生物学 構造生物学とは

背景:

  • 高級RNA構造,特に柔軟なドメインと折り畳み可能な中間物質を分析することは困難です.
  • 既存の方法は,機能のために構造的柔軟性を要求するRNAと戦っています.
  • 正確なRNA三次構造モデリングは,RNAの機能を理解するために不可欠です.

研究 の 目的:

  • RNAの三次構造をモデリングするための簡潔で正確なアプローチを開発する.
  • 柔軟なRNAのための従来の構造的方法の限界を克服するために.
  • RNAの折りたたみに関する事前の仮定なしに構造の決定を可能にするために.

主な方法:

  • 二次構造のSHAPE化学を含む,簡単なRNA構造探査実験を活用する.
  • 配列指向の割れ分剤を用いて,残留物間距離情報を生成する.
  • 実験データを高速で粗い粒子の離散分子動力学アルゴリズムで解釈する.
  • それぞれのRNAヌクレオチドを偽原子 (リン酸,リボース,核塩基) で表す.

主要な成果:

  • 酵母 tRNAの塩基対の位置を4 Åの根-平方平均偏差 (rmsd) にまで精製しました.
  • 既存の構造情報なしで,高解像度の構造的精錬を達成しました.
  • ユーザの介入なしに解像度と速度に最適化された計算アルゴリズムを実証した.

結論:

  • このブレンドされた実験的および計算的アプローチは,RNA構造モデリングのための強力な新しいツールを提供します.
  • この方法は,多様な,機能的に重要なRNAsのネイティブのようなモデルを生成する可能性を秘めています.
  • これは,従来の構造生物学技術では扱えないRNAを研究するための新しいアプローチの必要性に対処しています.