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Updated: Jun 25, 2026

PCR Mutagenesis, Cloning, Expression, Fast Protein Purification Protocols and Crystallization of the Wild Type and Mutant Forms of Tryptophan Synthase
09:31

PCR Mutagenesis, Cloning, Expression, Fast Protein Purification Protocols and Crystallization of the Wild Type and Mutant Forms of Tryptophan Synthase

Published on: September 26, 2020

人間のトリプトファン・ダイオキシゲナーゼにおける基板タンパク質相互作用:H76の重要な役割

Dipanwita Batabyal1, Syun-Ru Yeh

  • 1Department of Physiology and Biophysics, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, New York 10461, usa.

Journal of the American Chemical Society
|February 13, 2009
PubMed
まとめ

ヒスティジン76 (H76) をヒトのトリプトファン2,3-二酸化原酵素 (hTDO) で調べたところ,活性サイトベースとしての重要な役割が明らかになった. H76SとH76Aの突然変異は,触媒と基板結合を妨害することによって,酵素活性を著しく低下させます.

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Anaerobic Protein Purification and Kinetic Analysis via Oxygen Electrode for Studying DesB Dioxygenase Activity and Inhibition08:31

Anaerobic Protein Purification and Kinetic Analysis via Oxygen Electrode for Studying DesB Dioxygenase Activity and Inhibition

Published on: October 3, 2018

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