リボソームプロファイリングを用いたニュクレオチド解像度による翻訳の全ゲノム分析 in vivo
Nicholas T Ingolia1, Sina Ghaemmaghami, John R S Newman
1Department of Cellular and Molecular Pharmacology, Howard Hughes Medical Institute, University of California, San Francisco, and California Institute for Quantitative Biosciences, San Francisco, CA 94158, USA. ingolia@cmp.ucsf.edu
Science (New York, N.Y.)
|February 14, 2009
まとめ
私たちは,タンパク質翻訳を全ゲノムで正確に追跡するためのリボソームプロファイリング方法を開発しました. この技術は,酵母におけるタンパク質レベルとストレス反応に影響を与える広範な翻訳制御を明らかにします.
科学分野:
- 分子生物学は分子生物学である.
- ゲノミクスゲノミクスとは
- プロテオミクス プロテオミクスは,プロテオミクスの
背景:
- タンパク質翻訳の測定は,mRNAの定量化に遅れをとっている.
- タンパク質合成のダイナミクスを理解することは,細胞機能にとって極めて重要です.
研究 の 目的:
- ゲノム全体の翻訳モニタリングのための高解像度リボソームプロファイリング戦略を提示する.
- 異なる条件下で芽生える酵母におけるトランスレーション制御を調査する.
主な方法:
- リボソームで保護されたmRNA断片のディープシーケンシング.
- 芽生えた酵母 (Saccharomyces cerevisiae) でリボソームプロファイリングの適用.
- 富と飢餓の条件下での翻訳の分析.
主要な成果:
- 定義されたタンパク質配列が,サブコドン解像度で翻訳される.
- タンパク質の豊富さとストレス反応に影響を与える広範な翻訳制御を特定しました.
- 延長中のリボソーム密度の変化を含む,明確なトランスレーション相を観察した.
- 非正規のコドンで調節されたイニシアーションが検出されました.
結論:
- リボソームプロファイリングは,ゲノム全体の翻訳を研究する際,前例のない精度を提供します.
- 翻訳制御は,細胞適応とタンパク質ホメオスタシスにおいて重要な役割を果たします.
- この方法は,高精度の翻訳研究のために様々な生物に適応できます.
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