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Regulated mRNA Transport02:22

Regulated mRNA Transport

In eukaryotes, transcription and translation are compartmentalized; an mRNA is first synthesized in the nucleus and then selectively transported to the cytoplasm for protein synthesis. Before transport, a pre-mRNA undergoes several steps of post-transcriptional modifications including splicing, 5' capping, and the addition of a poly-adenine tail. Various proteins bind to the pre-mRNA during these modifications. The mRNA transport takes place with the help of multiple proteins playing specific...
Regulated mRNA Transport02:22

Regulated mRNA Transport

In eukaryotes, transcription and translation are compartmentalized; an mRNA is first synthesized in the nucleus and then selectively transported to the cytoplasm for protein synthesis. Before transport, a pre-mRNA undergoes several steps of post-transcriptional modifications including splicing, 5' capping, and the addition of a poly-adenine tail. Various proteins bind to the pre-mRNA during these modifications. The mRNA transport takes place with the help of multiple proteins playing specific...
Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...
Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the addition of a...
Chromatin Position Affects Gene Expression02:35

Chromatin Position Affects Gene Expression

Chromatin is the massive complex of DNA and proteins packaged inside the nucleus. The complexity of chromatin folding and how it is packaged inside the nucleus greatly influences  access to genetic information. Generally, the nucleus' periphery is considered transcriptionally repressive, while the cell's interior is considered a transcriptionally active area. 
Topologically Associated Domains (TADs)
The 3-dimensional positioning of chromatin in the nucleus influences the timing and level of...

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Updated: Jun 25, 2026

Production of Xenopus tropicalis Egg Extracts to Identify Microtubule-associated RNAs
10:01

Production of Xenopus tropicalis Egg Extracts to Identify Microtubule-associated RNAs

Published on: June 27, 2013

mRNAの局所化:空間的な次元における遺伝子発現.

Kelsey C Martin1, Anne Ephrussi

  • 1Department of Psychiatry and Biobehavioral Sciences, Semel Institute for Neuroscience and Human Behavior, University of California, Los Angeles, CA 90095-1737, USA. kcmartin@mednet.ucla.edu

Cell
|February 26, 2009
PubMed
まとめ
この要約は機械生成です。

メッセンジャーRNA (mRNA) は,遺伝子発現を制御するために特定の細胞部位に導かれます. このレビューでは,RNA要素,タンパク質,および粒子が,正確な細胞調節のためのmRNA輸送をどのように可能にするかを調査します.

さらに関連する動画

Mining Spatial Transcriptomics Datasets using DeepSpaceDB
10:16

Mining Spatial Transcriptomics Datasets using DeepSpaceDB

Published on: September 5, 2025

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection
09:19

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Published on: July 6, 2022

関連する実験動画

Last Updated: Jun 25, 2026

Production of Xenopus tropicalis Egg Extracts to Identify Microtubule-associated RNAs
10:01

Production of Xenopus tropicalis Egg Extracts to Identify Microtubule-associated RNAs

Published on: June 27, 2013

Mining Spatial Transcriptomics Datasets using DeepSpaceDB
10:16

Mining Spatial Transcriptomics Datasets using DeepSpaceDB

Published on: September 5, 2025

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection
09:19

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Published on: July 6, 2022

科学分野:

  • 分子生物学は分子生物学である.
  • 細胞生物学 細胞生物学
  • 遺伝学 遺伝学とは

背景:

  • mRNAの局所化は,空間的および時間的遺伝子発現制御の重要なメカニズムです.
  • mRNAの有意な部分は,特定の細胞プラズマ領域に蓄積することが知られている.
  • mRNAの輸送を理解することは,細胞の機能と発達を理解するために不可欠です.

研究 の 目的:

  • 細胞内のmRNAの局所化を制御するメカニズムを見直す.
  • cis作用要素,RNA結合タンパク質,mRNA粒子の役割を強調する.
  • mRNA輸送における分子モーターと細胞骨格経路の関与について議論する.

主な方法:

  • mRNAの局所化に関する最近の研究の文献レビュー.
  • RNA輸送に関与する分子機構の分析.
  • RNA結合タンパク質と粒子の形成に関する情報の合成.

主要な成果:

  • mRNAの局所化を指向するために重要なcis作用RNA要素の識別.
  • mRNA輸送を媒介するRNA結合タンパク質の役割の解明.
  • mRNAの粒子の組成と,細胞骨格に沿ったモーターに依存した移動の説明.

結論:

  • mRNAの局所化は,細胞機能に不可欠な洗練されたプロセスです.
  • RNA要素,タンパク質,および粒子の相互作用により,標的型mRNAの配送が容易になります.
  • mRNAの輸送メカニズムに関するさらなる研究は,様々な生物学的プロセスや疾患に関する洞察を明らかにすることができます.