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Updated: Jun 24, 2026

How to Quantify the Fraction of Photoactivated Fluorescent Proteins in Bulk and in Live Cells
11:03

How to Quantify the Fraction of Photoactivated Fluorescent Proteins in Bulk and in Live Cells

Published on: January 7, 2019

光活性化可能な緑色光タンパク質の構造とメカニズム

J Nathan Henderson1, Rinat Gepshtein, Josef R Heenan

  • 1Institute of Molecular Biology and Department of Physics, University of Oregon, Eugene, Oregon 97403-1229, USA.

Journal of the American Chemical Society
|March 13, 2009
PubMed
まとめ

光活性化可能な緑色光タンパク質 (PA-GFP) の結晶構造は,UV誘発のGlu222デカルボキシル化が光活性化を引き起こすことを示しています. このメカニズムは,安定化変異と組み合わせて,野生型GFPの100倍以上のコントラストを高めます.

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Engineering 'Golden' Fluorescence by Selective Pressure Incorporation of Non-canonical Amino Acids and Protein Analysis by Mass Spectrometry and Fluorescence11:51

Engineering 'Golden' Fluorescence by Selective Pressure Incorporation of Non-canonical Amino Acids and Protein Analysis by Mass Spectrometry and Fluorescence

Published on: April 27, 2018

Residue-Specific Exchange of Proline by Proline Analogs in Fluorescent Proteins: How "Molecular Surgery" of the Backbone Affects Folding and Stability10:31

Residue-Specific Exchange of Proline by Proline Analogs in Fluorescent Proteins: How "Molecular Surgery" of the Backbone Affects Folding and Stability

Published on: February 3, 2022

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