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Updated: Jun 24, 2026

The Importance of Correct Protein Concentration for Kinetics and Affinity Determination in Structure-function Analysis
19:16

The Importance of Correct Protein Concentration for Kinetics and Affinity Determination in Structure-function Analysis

Published on: March 17, 2010

タンパク質G B1ドメインのライシンへの塩橋の特徴化

Jennifer H Tomlinson1, Saif Ullah, Poul Erik Hansen

  • 1Department of Molecular Biology and Biotechnology, University of Sheffield, Firth Court, Western Bank, Sheffield, S10 2TN UK.

Journal of the American Chemical Society
|March 14, 2009
PubMed
まとめ

核磁共振 (NMR) 研究は,結晶構造の証拠にもかかわらず,タンパク質Gの表面ライシンが有意な塩橋を形成しないことを明らかにしています. 陽子交換率は,塩橋の安定化ではなく,炭酸塩酸塩酸塩触媒を示唆しています.

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