アシンメトリックな細胞分裂中のサラエンドソームの方向性デルタとノッチの密輸
F Coumailleau1, M Fürthauer, J A Knoblich
1Department of Biochemistry, University of Geneva, 30 Quai Ernest-Ansermet, 1211 Geneva 4, Switzerland.
Nature
|March 20, 2009
まとめ
新しいメカニズムは,細胞分裂の前にノッチ信号がどのように導かれるかを明らかにしています. 内化されたノッチおよびデルタタンパク質は,非対称的に子細胞に輸送され,フライの感覚器官の発達におけるシグナリング結果をバイアスします.
科学分野:
- 発達生物学 発達生物学について
- 細胞生物学 細胞生物学
- 分子生物学は分子生物学である.
背景:
- ノッチ・シグナリングは,非対称的な細胞分裂後の細胞運命を決定するのに極めて重要です.
- ディレクショナル・ノッチ・シグナリングは,デルタエフェクターとノッチエフェクターによる微分エンドサイトーシスに依存しています.
- 以前のモデルは,子細胞 (pIIaとpIIb) の内細胞化に焦点を当てていた.
研究 の 目的:
- 方向ノッチ信号の新しいメカニズムを解明する.
- 細胞分裂中のNotchとDeltaの細胞内密輸の役割を調査する.
- 信号バイアスが非対称性エンドサイトーシス以前にどのように確立されるかを理解する.
主な方法:
- 感覚器官前駆体 (SOP) の内部化されたデルタおよびノッチタンパク質の追跡.
- SOPミトーシス中のサラ陽性エンドソームへのタンパク質密輸の分析.
- 子細胞 (pIIaおよびpIIb) のノッチ割れと活性化の評価.
主要な成果:
- 内部化されたデルタとノッチのトラフィックをサラエンドソームに分割SOP内で.
- ノッチとデルタを含むサラエンドゾームは,中央のスパインドルへ,それからpIIa子細胞へ移動する.
- 隙間割れは,サラエンドソーム内のpIIaで発生し,ガンマ分泌酵素とデルタ内部化に依存しています.
結論:
- 新しいメカニズムは,内蔵されたリガンドと受容体の非対称な取引を通じて,ノッチ信号を誘導します.
- サラエンドソームのこの前代謝前非対称的標的化は,ピリア細胞に向かってノッチ信号をバイアスする.
- このメカニズムは,子細胞の微分性エンドサイトーシス前にシグナル伝達非対称性を確立し,ハエの感覚器官の発達に影響を与えます.
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