Ezh2は,組織特異性幹細胞の段階的な分化のための遺伝子発現をオーケストラします
Elena Ezhkova1, H Amalia Pasolli, Joel S Parker
1Howard Hughes Medical Institute, Laboratory of Mammalian Cell Biology and Development, The Rockefeller University, New York, NY 10065, USA.
Cell
|March 24, 2009
まとめ
ポリコンブ・レプレッサー複合体 (PRC) は,表皮の原始細胞の分化を調節する. PRCの成分であるEzh2は,特定の遺伝子を抑制することで,原始細胞の増殖を維持し,分化タイミングを制御します.
科学分野:
- 発達生物学 発達生物学とは
- エピジェネティクス エピジェネティクス
- 幹細胞生物学 幹細胞生物学とは
背景:
- ポリコンブ抑制複合体 (PRC) は,胚性幹細胞の微分化のレギュレータとして知られています.
- 発達中の組織における多効性原始体分化制御におけるPRCの役割は十分に理解されていません.
研究 の 目的:
- 皮膚の原始細胞の分化におけるPRCの重要な成分であるEzh2の役割を調査する.
- Ezh2が祖先の増殖と分化タイミングを制御するメカニズムを解明する.
主な方法:
- 胚および産後発達中の表皮原体におけるEzh2発現の分析.
- Ink4A-Ink4BロカスとAP1トランスクリプションアクティベーターの募集にEzh2の影響を調査する.
- 組織制限性幹細胞における表遺伝的変異の時間的・空間的制御を研究する.
主要な成果:
- エピダーマルの原始体におけるEzh2発現は,分化と減少した増殖活動により減少する.
- Ezh2は,Ink4A-Ink4Bの位置を抑制し,基礎原始体の増殖可能性を制御する.
- Ezh2は,分化遺伝子への早期のAP1の採用を防止し,表皮分化の速度を温める.
結論:
- PRCsは,Ezh2を通じて,組織に制限された幹細胞に対する時的,空間的な表遺伝子制御を行います.
- PRCは,グローバルに差異化を抑制することによって,祖先の増殖能力を維持します.
- 中国では,ターミナル差異化プログラムを段階的に選択的に確立しています.
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