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関連する概念動画

Clathrin Coated Vesicles01:12

Clathrin Coated Vesicles

Clathrin-coated vesicles use endocytosis to transport receptors and lysosomal hydrolases from the Golgi to the lysosome in the late secretory pathway. Clathrin-mediated endocytosis was the first described endocytic process, and Clathrin-coated vesicles remain one of the most well-studied transport vesicles. The molecular machinery that generates clathrin-coated vesicles comprises over 50 proteins that precisely coordinate vesicle formation. Cell surface receptors concentrated in indented sites...
Fluid Mosaic Model01:19

Fluid Mosaic Model

Scientists identified the plasma membrane in the 1890s and its principal chemical components (lipids and proteins) by 1915. The model for plasma membrane structure, proposed in 1935 by Hugh Davson and James Danielli, was the first model to be widely accepted in the scientific community. The model was based on the plasma membrane's "railroad track" appearance in early electron micrographs. Davson and Danielli theorized that the plasma membrane's structure resembled a sandwich with the analogy of...
COP Coated Vesicles00:59

COP Coated Vesicles

Membrane-enclosed structures called vesicles transport proteins and lipids across the cell. The vesicles derive their cargo from the plasma membrane, Golgi, ER, or endosome. Coated vesicles are spherical, protein-coated carriers with a 50–100 nm diameter that mediate bidirectional transport between the ER and the Golgi. The distribution of proteins between the ER and Golgi complex is dynamic and is maintained by different coated vesicles. Their formation is driven by the assembly of different...
Membrane Carbohydrates01:30

Membrane Carbohydrates

The plasma membrane is a dynamic barrier composed of lipids, proteins, and carbohydrates. It is the epicenter of many cellular processes required for cell growth and survival. Carbohydrates have unique structural and chemical properties that help the plasma membrane to carry out its functions effectively.
Membrane carbohydrates do not have any hydrophobic region and are exclusively located on the cell's outer surface. The addition of sugar molecules or glycosylation of proteins happens in...
Pinching-off of Coated Vesicles01:32

Pinching-off of Coated Vesicles

Vesicle budding is orchestrated by distinct cytosolic proteins such as adaptor proteins, coat proteins, and GTPases. To initiate vesicle budding, membrane-bending proteins containing crescent-shaped BAR domains bind to the lipid heads in the bilayer and distort the membrane to form a protein-coated vesicle bud. Adaptors proteins such as AP2 for clathrin-coated vesicles can nucleate on the deformed membrane. Finally, coat proteins such as clathrin or COPI and COPII assemble into a coat forming...

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関連する実験動画

Updated: Jun 24, 2026

Lipid Vesicle-mediated Affinity Chromatography using Magnetic Activated Cell Sorting (LIMACS): a Novel Method to Analyze Protein-lipid Interaction
07:33

Lipid Vesicle-mediated Affinity Chromatography using Magnetic Activated Cell Sorting (LIMACS): a Novel Method to Analyze Protein-lipid Interaction

Published on: April 26, 2011

炭水化物改変のカタニオニンベジクル:二重層インターフェースで多価結合を検出する.

Glen B Thomas1, Lenea H Rader, Juhee Park

  • 1Department of Chemistry and Biochemistry, University of Maryland, College Park, Maryland 20742, USA.

Journal of the American Chemical Society
|March 28, 2009
PubMed
まとめ
この要約は機械生成です。

研究者は,糖分分子の分布がタンパク質結合にどのように影響するかを研究するために,機能化された膀を合成しました. 彼らは,この分布を制御することが結合運動に影響し,膀の集積につながり,インターフェースの分子相互作用の洞察を提供することを発見しました.

さらに関連する動画

Assembly of Cell Mimicking Supported and Suspended Lipid Bilayer Models for the Study of Molecular Interactions
12:18

Assembly of Cell Mimicking Supported and Suspended Lipid Bilayer Models for the Study of Molecular Interactions

Published on: August 3, 2021

関連する実験動画

Last Updated: Jun 24, 2026

Lipid Vesicle-mediated Affinity Chromatography using Magnetic Activated Cell Sorting (LIMACS): a Novel Method to Analyze Protein-lipid Interaction
07:33

Lipid Vesicle-mediated Affinity Chromatography using Magnetic Activated Cell Sorting (LIMACS): a Novel Method to Analyze Protein-lipid Interaction

Published on: April 26, 2011

Assembly of Cell Mimicking Supported and Suspended Lipid Bilayer Models for the Study of Molecular Interactions
12:18

Assembly of Cell Mimicking Supported and Suspended Lipid Bilayer Models for the Study of Molecular Interactions

Published on: August 3, 2021

科学分野:

  • 超分子化学 超分子化学
  • 生物物理化学 生物物理化学
  • マテリアルサイエンス 材料科学

背景:

  • インタフェースにおける分子相互作用を理解することは,先進的な材料と薬物投与システムの開発に不可欠です.
  • 膀の表面上のリガンドの空間的配置は,多価タンパク質との結合に影響する.
  • カタニオンの膀は,これらのインターフェイス現象を研究するための調整可能なプラットフォームを提供します.

研究 の 目的:

  • 表面機能化された,負の電荷を持つカタニオニンベシクルを合成し,特徴づけること.
  • レクチン結合運動に対するグリココンジュガート分布の影響を調査する.
  • リガンド分離距離と多価結合イベントの関係を探求する.

主な方法:

  • 表面機能化されたカタニオニク小胞の合成と特徴付け.
  • O-リンクおよびN-リンクのグルコシル結合剤とレクチンを用いた拘束的研究.
  • 形態学的分析のための冷凍伝送電子顕微鏡 (冷凍-TEM).

主要な成果:

  • 膀膜におけるグリココンジュガートの分布は,化学的構造を変更することによって制御することができます.
  • O-リンクコンジュガットは非相互作用リガンド結合運動を示し,N-リンクコンジュガットは相互作用リガンドまたはクラスターリガンドを示した.
  • Cryo-TEMでは,コンカナヴァリンAとの結合により,リガンド密度に依存する膀の多層化が明らかになりました.

結論:

  • 膀の表面での制御されたリガンド分布は,多価結合相互作用の研究を可能にします.
  • この発見は,有効な結合部位分離と臨界リガンド密度を決定するための方法を提供する.
  • 表面機能化された膀は,基本的な結合現象と物質の自己組み立ての研究に有望である.