Jove
Visualize
お問い合わせ
JoVE
x logofacebook logolinkedin logoyoutube logo
JoVEについて
概要リーダーシップブログJoVEヘルプセンター
著者向け
出版プロセス編集委員会範囲と方針査読よくある質問投稿
図書館員向け
推薦の声購読アクセスリソース図書館諮問委員会よくある質問
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experimentsアーカイブ
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教員リソースセンター教員サイト
利用規約
プライバシーポリシー
ポリシー

関連する概念動画

Attachment of Sister Chromatids02:57

Attachment of Sister Chromatids

As cells progress into mitosis, the nuclear envelope breaks down, and the condensed chromosomes are exposed to the array of bipolar microtubules of the mitotic spindle. The kinetochore, a large, disc-shaped protein complex, is present at the centromere region of the sister chromatids and acts as a binding site for the microtubules.  Usually, the plus-end of a single microtubule is embedded within the kinetochore. However, some kinetochores first establish lateral contact with the side-wall of a...
Attachment of Sister Chromatids02:57

Attachment of Sister Chromatids

As cells progress into mitosis, the nuclear envelope breaks down, and the condensed chromosomes are exposed to the array of bipolar microtubules of the mitotic spindle. The kinetochore, a large, disc-shaped protein complex, is present at the centromere region of the sister chromatids and acts as a binding site for the microtubules.  Usually, the plus-end of a single microtubule is embedded within the kinetochore. However, some kinetochores first establish lateral contact with the side-wall of a...
Histone Variants at the Centromere02:30

Histone Variants at the Centromere

Histone variants are the histone proteins with structural and sequence variations. These variants may be regarded as “mutant” forms that replace their canonical histone counterparts in the nucleosomes. Specific post-translational modifications on the histone variants enable further chromatin complexity and regulate tissue-specific gene expression. The most common histone variants are from histone H2A, H2B, and linker histone H1 families. However, several variants of histone H3 variants are also...
Cohesins02:20

Cohesins

Cohesin protein complexes are a molecular glue that holds two sister chromatids together. They play an important role both in mitosis and meiosis. In mitosis, all cohesin complexes present on the chromosomes are removed before the start of the anaphase stage.
Cohesin complexes in Meiotic Division
Meiosis involves two distinct rounds of chromosomal segregation and cell divisions— Meiosis I followed by Meiosis II – producing four daughter cells. Meiosis I includes the separation of homologous...
Cohesins02:20

Cohesins

Cohesin protein complexes are a molecular glue that holds two sister chromatids together. They play an important role both in mitosis and meiosis. In mitosis, all cohesin complexes present on the chromosomes are removed before the start of the anaphase stage.
Cohesin complexes in Meiotic Division
Meiosis involves two distinct rounds of chromosomal segregation and cell divisions— Meiosis I followed by Meiosis II – producing four daughter cells. Meiosis I includes the separation of homologous...
Chromosome Structure02:40

Chromosome Structure

A functional eukaryotic chromosome must contain three elements: a centromere, telomeres, and numerous origins of replication.
The centromere is a DNA sequence that links sister chromatids. This is also where kinetochores, protein complexes to which spindle microtubules attach, are constructed after the chromosome is replicated. The kinetochores allow the spindle microtubules to move the chromosomes within the cell during cell division.
Telomeres consist of non-coding repetitive nucleotide...

こちらも読む

関連記事

共著者、ジャーナル、引用グラフによってこの研究に関連する記事。

並び替え
Same author

Topical β-Blocker as a Novel Treatment Option for Periungual Pyogenic Granuloma: A Retrospective Study of Lesion Size Reduction and Pain Improvement.

The Journal of dermatology·2026
Same author

Protection and deprotection of the Rec8 cohesin complex during meiosis.

Cell reports·2026
Same author

Interaction Between Rec8 and Mis4 Is Required for Axis-Loop Chromatin Formation and Homologous Chromosome Recombination During Meiosis.

Genes to cells : devoted to molecular & cellular mechanisms·2026
Same author

Shugoshin holds the potential to inhibit APC/C and thereby prevents separase activation.

International journal of biological macromolecules·2026
Same author

Interfacility Transfer and Mortality in Patients With Type A Acute Aortic Dissection　- Analysis of the Tokyo Acute Aortic Super-Network Database.

Circulation reports·2026
Same author

Ultrafine bubble water irrigation promotes soil particle fragmentation and reduces soil hardness in paddy fields.

Scientific reports·2025

関連する実験動画

Updated: Jun 24, 2026

Immunofluorescence Analysis of Endogenous and Exogenous Centromere-kinetochore Proteins
05:35

Immunofluorescence Analysis of Endogenous and Exogenous Centromere-kinetochore Proteins

Published on: March 3, 2016

キネトコア幾何学は,セントロメア内の凝結によって定義される.

Takeshi Sakuno1, Kenji Tada, Yoshinori Watanabe

  • 1Laboratory of Chromosome Dynamics, Institute of Molecular and Cellular Biosciences, University of Tokyo, Yayoi, Tokyo 113-0032, Japan.

Nature
|April 17, 2009
PubMed
まとめ

セントロメア凝結は,細胞分裂中のキネトコア指向を調節する. コア・セントロメア・コヘシオンはモノ・オリエンテーションを促進し,周辺・セントロメア・コヘシオンは分裂酵母におけるバイ・オリエンテーションを促進する.

科学分野:

  • 細胞生物学 細胞生物学
  • 遺伝学 遺伝学とは
  • 分子生物学は分子生物学である.

背景:

  • マイクロチューブルは,細胞分裂時にキネトコアを通して染色体を捕らえます.
  • ミトーシスには双極結合があり,メイオシスIには単極結合がある.
  • キネトコア幾何学は染色体指向に不可欠ですが,その調節は十分に理解されていません.

研究 の 目的:

  • Schizosaccharomyces pombeにおけるセントロメア凝結とキネトコア指向の関連性を調査する.
  • キネトコア幾何学調節の分子基礎を解明する.

主な方法:

  • セントロメリック・コーヘションを視覚化するための新しい戦略を開発した.
  • キネトコアの幾何学を操作するために人工の鎖を導入した.
  • 分裂酵母 (Schizosaccharomyces pombe) をモデル生物として利用した.

主要な成果:

  • コア・セントロメアにおける凝結が,キネトコア・モノ・オリエンテーションを誘導することを実証した.
  • 周辺・中央帯域における結束が双方向性を促進することを示した.
  • セントロメア構造と染色体結合のダイナミクスとの関係を確立した.

さらに関連する動画

Studying Mitotic Checkpoint by Illustrating Dynamic Kinetochore Protein Behavior and Chromosome Motion in Living Drosophila Syncytial Embryos
13:59

Studying Mitotic Checkpoint by Illustrating Dynamic Kinetochore Protein Behavior and Chromosome Motion in Living Drosophila Syncytial Embryos

Published on: June 14, 2012

Live Imaging Characterization of Centromere Movements During Male Meiotic Prophase in Arabidopsis thaliana
07:18

Live Imaging Characterization of Centromere Movements During Male Meiotic Prophase in Arabidopsis thaliana

Published on: October 24, 2025

関連する実験動画

Last Updated: Jun 24, 2026

Immunofluorescence Analysis of Endogenous and Exogenous Centromere-kinetochore Proteins
05:35

Immunofluorescence Analysis of Endogenous and Exogenous Centromere-kinetochore Proteins

Published on: March 3, 2016

Studying Mitotic Checkpoint by Illustrating Dynamic Kinetochore Protein Behavior and Chromosome Motion in Living Drosophila Syncytial Embryos
13:59

Studying Mitotic Checkpoint by Illustrating Dynamic Kinetochore Protein Behavior and Chromosome Motion in Living Drosophila Syncytial Embryos

Published on: June 14, 2012

Live Imaging Characterization of Centromere Movements During Male Meiotic Prophase in Arabidopsis thaliana
07:18

Live Imaging Characterization of Centromere Movements During Male Meiotic Prophase in Arabidopsis thaliana

Published on: October 24, 2025

結論:

  • セントロメア凝結は,キネトコアの幾何学と向きを調節する上で重要な役割を果たします.
  • このメカニズムは,キネトコアの幾何学的な制御のための一般的な原理を表すかもしれません.
  • この発見は,電圧依存型方向転換装置の既存のモデルを補完しています.