マウスにおける生殖細胞系統の特定のためのシグナル伝達原理

Yasuhide Ohinata¹, Hiroshi Ohta, Mayo Shigeta

¹Center for Developmental Biology, RIKEN Kobe Institute, Minatojima-Minamimachi, Chuo-ku, Japan.

Cell

|May 5, 2009

まとめ

ネズミの生殖細胞の運命は,外胚内皮からのBmp4信号によって誘発され,前内皮内皮によって敵対されます. この研究では,哺乳類の生殖細胞の系統をインビトロで再構成し,精子発生と生育を可能にします.

科学分野:

発達生物学発達生物学について
生殖生物学生殖生物学
幹細胞科学幹細胞科学とは

背景:

生殖細胞系統の仕様は,遺伝性にとって極めて重要です.
マウスにおける生殖細胞誘導の分子機構は不明である.

研究の目的:

エピブラストにおける生殖細胞の運命を左右するシグナル伝達経路を解明する.
哺乳類の生殖細胞の系統をin vitroで再構成する方法を確立する.

主な方法:

血清フリーで定義された細胞培養を用いた.
Bmp4,Bmp8b,およびWnt3のシグナル伝達の役割を調査しました.
重要な転写レギュレータ (Blimp1, Prdm14) の発現を分析した.

主要な成果:

生殖細胞の運命は,Bmp4のシグナル伝達による直接的な結果であり,前部内皮内皮によって敵対されます.
Bmp8bは前頭内皮内皮の発達を制限し,Bmp4の信号伝達を強化する.
エピブラスト細胞は,Bmp1とPrdm14をBmp4に反応して均一に発現させ,エピジェネティック再プログラムを含む生殖細胞の特性を獲得した.
誘導された細胞は精子発生と子孫の生育に寄与した.

結論:

生殖細胞の特異化のための重要な信号伝達原理を特定した.
哺乳類の生殖細胞系統を再構成するための堅実なインビトロ戦略を示した.

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